Evidence map›Paper›PMID 34830492›Full record

ArticleInternational journal of molecular sciences2021

Tracing CRISPR/Cas12a Mediated Genome Editing Events in Apple Using High-Throughput Genotyping by PCR Capillary Gel Electrophoresis.

Susan Schröpfer, Henryk Flachowsky

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Susan SchröpferJulius Kühn Institute (JKI)-Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, 01326 Dresden, Germany.ORCID 0000-0003-3249-2161
Henryk FlachowskyJulius Kühn Institute (JKI)-Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, 01326 Dresden, Germany.ORCID 0000-0003-1440-1895
Julius Kühn-Institut · DE

Funding

Federal Ministry of Education and Research 031B0532
6 · The paper itself

Abstract

The use of the novel CRISPR/Cas12a system is advantageous, as it expands the possibilities for genome editing (GE) applications due to its different features compared to the commonly used CRISPR/Cas9 system. In this work, the CRISPR/Cas12a system was applied for the first time to apple to investigate its general usability for GE applications. Efficient guide RNAs targeting different exons of the endogenous reporter gene

Indexed as

Gene EditingCRISPR-Cas SystemsElectrophoresis, CapillaryGenotypeGenotyping TechniquesMalusMutationamplicon deep sequencingCpf1deletionfragment length analysisMalus domesticaMdPDSmutation

Identifiers

PMID34830492
PMCPMC8619667
OpenAlexW3217414094

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.