Evidence map›Paper›PMID 34830426›Full record

ArticleInternational journal of molecular sciences2021

Using Multiplexed CRISPR/Cas9 for Suppression of Cotton Leaf Curl Virus.

Barkha Binyameen, Zulqurnain Khan, Sultan Habibullah Khan, Aftab Ahmad, Nayla Munawar, Muhammad Salman Mubarik, Hasan Riaz, Zulfiqar Ali, Asif Ali Khan, Alaa T Qusmani and 2 more

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 30 citations in OpenAlex.

  1. Article
  2. Review
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  5. A comprehensive review onFrontiers in genetics · 2024
    Review
  6. Review
  7. Review
  8. LbCas12a mediated suppression ofFrontiers in plant science · 2023
    Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 4 countries.

Barkha BinyameenInstitute of Plant Breeding and Biotechnology, MNS University of Agriculture Multan, Old Shujabad Road, Multan 60000, Pakistan.
Zulqurnain KhanInstitute of Plant Breeding and Biotechnology, MNS University of Agriculture Multan, Old Shujabad Road, Multan 60000, Pakistan.ORCID 0000-0002-6910-7389
Sultan Habibullah KhanCotton Biotechnology Lab, Center for Advanced Studies in Agriculture and Food Security, University of Agriculture, Faisalabad 38040, Pakistan.
Aftab AhmadCotton Biotechnology Lab, Center for Advanced Studies in Agriculture and Food Security, University of Agriculture, Faisalabad 38040, Pakistan.ORCID 0000-0002-2792-9771
Nayla MunawarDepartment of Chemistry, College of Sciences, United Arab Emirates University, Al-Ain 15551, United Arab Emirates.
Muhammad Salman MubarikCotton Biotechnology Lab, Center for Advanced Studies in Agriculture and Food Security, University of Agriculture, Faisalabad 38040, Pakistan.ORCID 0000-0001-6522-4660
Hasan RiazInstitute of Plant Protection, MNS University of Agriculture Multan, Old Shujabad Road, Multan 60000, Pakistan.
Zulfiqar AliInstitute of Plant Breeding and Biotechnology, MNS University of Agriculture Multan, Old Shujabad Road, Multan 60000, Pakistan.ORCID 0000-0003-1228-3338
Asif Ali KhanInstitute of Plant Breeding and Biotechnology, MNS University of Agriculture Multan, Old Shujabad Road, Multan 60000, Pakistan.
Alaa T QusmaniBiology Department, Al-Jumum University College, Umm Al-Qura University, Makkah 21961, Saudi Arabia.
Kamel A Abd-ElsalamPlant Pathology Research Institute, Agricultural Research Center (ARC), 9-Gamaa Str., Giza 12619, Egypt.ORCID 0000-0002-4042-4022
Sameer H QariDepartment of Biology, Genetics and Molecular Biology Central Laboratory (GMCL), Aljumum University College, Umm Al-Qura University, Makkah 21961, Saudi Arabia.ORCID 0000-0001-9424-5217
Muhammad Nawaz Shareef University of Agriculture · PKUniversity of Agriculture Faisalabad · PKUmm al-Qura University · SAAgricultural Research Center · EGUnited Arab Emirates University · AE

Funding

Higher Education Commission SRGP-2229
6 · The paper itself

Abstract

In recent decades, Pakistan has suffered a decline in cotton production due to several factors, including insect pests, cotton leaf curl disease (CLCuD), and multiple abiotic stresses. CLCuD is a highly damaging plant disease that seriously limits cotton production in Pakistan. Recently, genome editing through CRISPR/Cas9 has revolutionized plant biology, especially to develop immunity in plants against viral diseases. Here we demonstrate multiplex CRISPR/Cas-mediated genome editing against CLCuD using transient transformation in

Indexed as

AgrobacteriumBegomovirusCRISPR-Cas SystemsDisease ResistanceGossypiumPlant DiseasesPlants, Genetically ModifiedSolanaceaeCLCuDcottonCRISPR/CasDsRedN. benthamianatransformationvirus inhibition

Identifiers

PMID34830426
PMCPMC8618328
OpenAlexW3216970734

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.