High-Throughput and Accurate Determination of Transgene Copy Number and Zygosity in Transgenic Maize: From DNA Extraction to Data Analysis.

Fang Liu, Jinkui Cheng, Xuhua Liu, Xi-Qing Wang

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Fang LiuCenter for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing 100193, China.ORCID 0000-0001-6692-5119

Jinkui ChengCenter for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing 100193, China.ORCID 0000-0002-7484-0497

Xuhua LiuDepartment of Mathematics, China Agricultural University, Beijing 100193, China.

Xi-Qing WangCenter for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing 100193, China.

Funding

the National Natural Science Foundation of China 31701988

6 · The paper itself

Abstract

It is vital to develop high-throughput methods to determine transgene copy numbers initially and zygosity during subsequent breeding. In this study, the target sequence of the previously reported endogenous reference gene

Indexed as

DNA Copy Number VariationsDNA PrimersGene DosagePlant BreedingPlants, Genetically ModifiedTransgenesZea maysDNA Primersdigital PCRhigh throughputmaize endogenous reference genesingle-nucleotide polymorphismSouthern blotTaqMan assaytransgene copy numberzygosity

Identifiers

PMID34830369

DOI10.3390/ijms222212487

PMCPMC8619409

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.