Evidence map›Paper›PMID 34830318›Full record

ArticleInternational journal of molecular sciences2021

Single Extracellular Vesicle Analysis Performed by Imaging Flow Cytometry and Nanoparticle Tracking Analysis Evaluate the Accuracy of Urinary Extracellular Vesicle Preparation Techniques Differently.

Marvin Droste, Tobias Tertel, Stefanie Jeruschke, Robin Dittrich, Evangelia Kontopoulou, Bernd Walkenfort, Verena Börger, Peter F Hoyer, Anja K Büscher, Basant K Thakur and 1 more

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed.

  1. Liquid biopsy in bladder cancer: towards precision oncology.Journal of translational medicine · 2026
    Review
  2. Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Uromodulin and the study of urinary extracellular vesicles.Journal of extracellular biology · 2024
    Review
  9. Review
  10. Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Marvin DrosteDepartment of Pediatrics II (Pediatric Nephrology), University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Tobias TertelInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.ORCID 0000-0001-8659-8610
Stefanie JeruschkeDepartment of Pediatrics II (Pediatric Nephrology), University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Robin DittrichInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Evangelia KontopoulouDepartment of Pediatrics III (Pediatric Hematology & Oncology), University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Bernd WalkenfortElectron Microscopy Unit, Imaging Center, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Verena BörgerInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.ORCID 0000-0002-8230-2218
Peter F HoyerDepartment of Pediatrics II (Pediatric Nephrology), University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Anja K BüscherDepartment of Pediatrics II (Pediatric Nephrology), University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Basant K ThakurDepartment of Pediatrics III (Pediatric Hematology & Oncology), University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Bernd GiebelInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.ORCID 0000-0003-2446-948X

Funding

Else Kröner-Fresenius-Stiftung D/106-21902
6 · The paper itself

Abstract

Small extracellular vesicles isolated from urine (uEVs) are increasingly recognized as potential biomarkers. Meanwhile, different uEV preparation strategies exist. Conventionally, the performance of EV preparation methods is evaluated by single particle quantification, Western blot, and electron microscopy. Recently, we introduced imaging flow cytometry (IFCM) as a next-generation single EV analysis technology. Here, we analyzed uEV samples obtained with different preparation procedures using nanoparticle tracking analysis (NTA), semiquantitative Western blot, and IFCM. IFCM analyses demonstrated that urine contains a predominant CD9

Indexed as

AdultBiomarkersChromatography, GelExtracellular VesiclesFemaleFlow CytometryHealthy VolunteersHumansMaleMolecular ImagingNanoparticlesTetraspanin 28Tetraspanin 29Tetraspanin 30UltrafiltrationUrinalysisBiomarkersCD63 protein, humanCD81 protein, humanCD9 protein, humanTetraspanin 28Tetraspanin 29Tetraspanin 30UMOD protein, humanUromodulinexosomesextracellular vesicle isolation methodsextracellular vesiclesimaging flow cytometrynanoparticle tracking analysisurine

Identifiers

PMID34830318
PMCPMC8620260

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.