Evidence map›Paper›PMID 34829808›Full record

ArticleBiomedicines2021

Chick Embryo Experimental Platform for Micrometastases Research in a 3D Tissue Engineering Model: Cancer Biology, Drug Development, and Nanotechnology Applications.

Anna Guller, Inga Kuschnerus, Vlada Rozova, Annemarie Nadort, Yin Yao, Zahra Khabir, Alfonso Garcia-Bennett, Liuen Olivia Liang, Aleksandra Polikarpova, Yi Qian and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedicines, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Anna GullerFaculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia.ORCID 0000-0002-8866-9838
Inga KuschnerusFaculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia.ORCID 0000-0002-4453-6849
Vlada RozovaFaculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia.ORCID 0000-0003-1032-4650
Annemarie NadortFaculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia.
Yin YaoElectron Microscope Unit, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW 2052, Australia.
Zahra KhabirFaculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia.
Alfonso Garcia-BennettFaculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia.ORCID 0000-0002-9097-0179
Liuen Olivia LiangFaculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia.
Aleksandra PolikarpovaARC Centre of Excellence for Nanoscale Biophotonics, Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
Yi QianMacquarie Medical School, Macquarie University, Sydney, NSW 2109, Australia.ORCID 0000-0001-6452-3300
Ewa M GoldysFaculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia.
Andrei V ZvyaginFaculty of Science and Engineering, Macquarie University, Sydney, NSW 2109, Australia.ORCID 0000-0001-8799-2257

Funding

ARC CENTRE FOR NANOSCALE BIOPHOTONICS CE14010003Macquarie University iMQRES scholarship for Anna GullerMacquarie University MQRF fellowship for Anna GullerRussian Science Foundation 21-74-30016SYDNEY VITAL TRANSLATIONAL CANCER CENTRE Seed grants for Anna GullerThe Ministry of Education and Science of the Russian Federation 075-15-2019-1927TOUR DE CURE Pioneer research grant for Annemarie Nadort, Anna Guller, Yi Qian, Ewa Goldys and Andrei Zvyagin
6 · The paper itself

Abstract

Colonization of distant organs by tumor cells is a critical step of cancer progression. The initial avascular stage of this process (micrometastasis) remains almost inaccessible to study due to the lack of relevant experimental approaches. Herein, we introduce an in vitro/in vivo model of organ-specific micrometastases of triple-negative breast cancer (TNBC) that is fully implemented in a cost-efficient chick embryo (CE) experimental platform. The model was built as three-dimensional (3D) tissue engineering constructs (TECs) combining human MDA-MB-231 cells and decellularized CE organ-specific scaffolds. TNBC cells colonized CE organ-specific scaffolds in 2-3 weeks, forming tissue-like structures. The feasibility of this methodology for basic cancer research, drug development, and nanomedicine was demonstrated on a model of hepatic micrometastasis of TNBC. We revealed that MDA-MB-231 differentially colonize parenchymal and stromal compartments of the liver-specific extracellular matrix (LS-ECM) and become more resistant to the treatment with molecular doxorubicin (Dox) and Dox-loaded mesoporous silica nanoparticles than in monolayer cultures. When grafted on CE chorioallantoic membrane, LS-ECM-based TECs induced angiogenic switch. These findings may have important implications for the diagnosis and treatment of TNBC. The methodology established here is scalable and adaptable for pharmacological testing and cancer biology research of various metastatic and primary tumors.

Indexed as

3D culturechick embryodoxorubicinlivermesoporous silica nanoparticlesmetastasismicrometastasistissue engineeringtriple-negative breast cancertumor models in vitro

Identifiers

PMID34829808
PMCPMC8615510

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.