ArticleFoods (Basel, Switzerland)2021
Development of a DNA Metabarcoding Method for the Identification of Bivalve Species in Seafood Products.
Article in Foods (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- A validated DNA metabarcoding approach for routine authentication of processed bivalves.Food chemistry. Molecular sciences · 2026Article
- Evolution and Vulnerability of the Global Ready-to-Eat Aquatic Products Trade Network: A Complex Network Analysis.Foods (Basel, Switzerland) · 2026Article
- Development of a Multiplex PCR Assay for Simultaneous Identification of Six Commercially Important Bivalves.Foods (Basel, Switzerland) · 2025Article
- Development of a DNA Metabarcoding Method for the Identification of Crustaceans (Foods (Basel, Switzerland) · 2025Article
- Harnessing genomic technologies for one health solutions in the tropics.Globalization and health · 2024Review
- Article
- Advances in detection technology for authentication of vegetable oils: A comprehensive review.Heliyon · 2024Review
- A next-generation sequencing approach for the detection of mixed species in canned tuna.Food chemistry: X · 2023Article
- Techniques for Food Authentication: Trends and Emerging Approaches.Foods (Basel, Switzerland) · 2023Article
- Development of a DNA Metabarcoding Method for the Identification of Insects in Food.Foods (Basel, Switzerland) · 2023Article
- Benchmarking and Validation of a Bioinformatics Workflow for Meat Species Identification Using 16S rDNA Metabarcoding.Foods (Basel, Switzerland) · 2023Article
- Identification of Fish Species and Targeted Genetic Modifications Based on DNA Analysis: State of the Art.Foods (Basel, Switzerland) · 2023Review
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Authors and funding
7 authors.
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Abstract
The production of bivalve species has been increasing in the last decades. In spite of strict requirements for species declaration, incorrect labelling of bivalve products has repeatedly been detected. We present a DNA metabarcoding method allowing the identification of bivalve species belonging to the bivalve families Mytilidae (mussels), Pectinidae (scallops), and Ostreidae (oysters) in foodstuffs. The method, developed on Illumina instruments, targets a 150 bp fragment of mitochondrial 16S rDNA. We designed seven primers (three primers for mussel species, two primers for scallop species and a primer pair for oyster species) and combined them in a triplex PCR assay. In each of eleven reference samples, the bivalve species was identified correctly. In ten DNA extract mixtures, not only the main component (97.0-98.0%) but also the minor components (0.5-1.5%) were detected correctly, with only a few exceptions. The DNA metabarcoding method was found to be applicable to complex and processed foodstuffs, allowing the identification of bivalves in, e.g., marinated form, in sauces, in seafood mixes and even in instant noodle seafood. The method is highly suitable for food authentication in routine analysis, in particular in combination with a DNA metabarcoding method for mammalian and poultry species published recently.
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