Evidence map›Paper›PMID 34827805›Full record

ArticleAnimals : an open access journal from MDPI2021

Identification of Reference Genes for Expression Studies in the Whole-Blood from Three Cattle Breeds under Two States of Livestock Weather Safety.

Kelly J Lozano-Villegas, Roy Rodríguez-Hernández, María P Herrera-Sánchez, Heinner F Uribe-García, Juan S Naranjo-Gómez, Rafael J Otero-Arroyo, Iang S Rondón-Barragán

Open access · goldAbstract read
In one paragraph

Article in Animals : an open access journal from MDPI, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Kelly J Lozano-VillegasImmunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Tolima, Colombia.ORCID 0000-0003-4525-8403
Roy Rodríguez-HernándezPoultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Tolima, Colombia.ORCID 0000-0002-4187-7381
María P Herrera-SánchezImmunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Tolima, Colombia.ORCID 0000-0002-1138-1625
Heinner F Uribe-GarcíaImmunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Tolima, Colombia.ORCID 0000-0001-9115-757X
Juan S Naranjo-GómezImmunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Tolima, Colombia.
Rafael J Otero-ArroyoGrupo de Investigación en Reproducción y Mejoramiento Genético Animal, Facultad de Ciencias Agropecuarias, Universidad de Sucre, Sincelejo 700001, Sucre, Colombia.
Iang S Rondón-BarragánImmunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Tolima, Colombia.ORCID 0000-0001-6980-892X
University of Tolima · COUniversity of Sucre · CO

Funding

Sistema General de Regalías de Colombia BPIN 2016000100026
6 · The paper itself

Abstract

Real-time PCR is widely used to study the relative abundance of mRNA due to its specificity, sensitivity, and repeatability quantification. However, relative quantification requires a reference gene, which should be stable in its expression, showing lower variation by experimental conditions or tissues. The aim of this study was to evaluate the stability of the expression of five commonly used reference genes

Indexed as

cattleenvironmental conditionsfarm animalsgenes normalizationheat stressmRNAreal-time PCR

Identifiers

PMID34827805
PMCPMC8614315
OpenAlexW3208229638

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.