Evidence map›Paper›PMID 34827131›Full record

ArticleBiology2021

A Digital PCR Assay to Quantify the Percentages of Hulled vs. Hulless Wheat in Flours and Flour-Based Products.

Caterina Morcia, Raffaella Bergami, Sonia Scaramagli, Chiara Delogu, Lorella Andreani, Paola Carnevali, Giorgio Tumino, Roberta Ghizzoni, Valeria Terzi

Open access · goldAbstract read
In one paragraph

Article in Biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Digital PCR for the Authentication of KAMUTFoods (Basel, Switzerland) · 2026
    Article
  2. A CombinatorialGenes · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Caterina MorciaConsiglio Per la Ricerca in Agricoltura e L'analisi Dell'economia Agraria-Centro di Ricerca Genomica e Bioinformatica (CREA-GB), Via San Protaso 302, 29017 Fiorenzuola d'Arda, PC, Italy.ORCID 0000-0001-5316-7435
Raffaella BergamiCoop Italia, Via del Lavoro, 6/8, I-40033 Casalecchio di Reno, BO, Italy.
Sonia ScaramagliCoop Italia, Via del Lavoro, 6/8, I-40033 Casalecchio di Reno, BO, Italy.ORCID 0000-0002-0007-2923
Chiara DeloguConsiglio Per la Ricerca in Agricoltura e L'analisi Dell'economia Agraria-Centro di Ricerca Difesa e Certificazione (CREA-DC), Via Emilia km 307, 26838 Tavazzano, LO, Italy.
Lorella AndreaniConsiglio Per la Ricerca in Agricoltura e L'analisi Dell'economia Agraria-Centro di Ricerca Difesa e Certificazione (CREA-DC), Via Emilia km 307, 26838 Tavazzano, LO, Italy.
Paola CarnevaliBarilla S.p.A., Via Mantova 166, I-43122 Parma, PR, Italy.
Giorgio TuminoPlant Breeding, Wageningen Plant Research, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.ORCID 0000-0002-6505-7652
Roberta GhizzoniConsiglio Per la Ricerca in Agricoltura e L'analisi Dell'economia Agraria-Centro di Ricerca Genomica e Bioinformatica (CREA-GB), Via San Protaso 302, 29017 Fiorenzuola d'Arda, PC, Italy.
Valeria TerziConsiglio Per la Ricerca in Agricoltura e L'analisi Dell'economia Agraria-Centro di Ricerca Genomica e Bioinformatica (CREA-GB), Via San Protaso 302, 29017 Fiorenzuola d'Arda, PC, Italy.ORCID 0000-0002-8006-6805
Centro di Ricerca per l’Orticoltura · ITCineca · ITBarilla (Italy) · ITWageningen University & Research · NL

Funding

European Union 817970
6 · The paper itself

Abstract

Several food products, made from hulled wheats, are now offered by the market, ranging from grains and pasta to flour and bakery products. The possibility of verifying the authenticity of wheat species used at any point in the production chain is relevant, in defense of both producers and consumers. A chip digital PCR assay has been developed to detect and quantify percentages of hulless (i.e., common and durum wheat) and hulled (i.e., einkorn, emmer and spelt) wheats in grains, flours and food products. The assay has been designed on a polymorphism in the miRNA172 target site of the AP2-5 transcription factor localized on chromosome 5A and involved in wheat spike morphogenesis and grain threshability. The assay has been evaluated even in a real-time PCR system to assess its applicability and to compare the analytical costs between dPCR and real-time PCR approaches.

Indexed as

authenticitydPCReinkornemmerhulled wheatsquantificationspeltTriticum dicoccumTriticum monococcumTriticum spelta

Identifiers

PMID34827131
PMCPMC8614899
OpenAlexW3213895177

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.