ArticlePlant molecular biology2022
NtCycB2 negatively regulates tobacco glandular trichome formation, exudate accumulation, and aphid resistance.
Article in Plant molecular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 44 citations in OpenAlex.
- From Model Plants toPlants (Basel, Switzerland) · 2026Review
- Functional Characterisation of HAIRPLUS (NtHAP) Genes Involved in Trichome Development and Specialised Metabolism of Tobacco.Plant biotechnology journal · 2026Article
- Cytological basis and key metabolite profiling of fresh tobacco leaves with different oiliness indices in Yunyan 87.Frontiers in plant science · 2026Article
- Transcriptomic and metabolomic analysis of tobacco responses to combined organic-chemical fertilization.BMC plant biology · 2025Article
- Insight into the genetic network governing long-stalked glandular trichome development in Nicotiana tabacum.Planta · 2025Article
- Genome-Wide Association Study and RNA-Seq Elucidate the Genetic Mechanisms Behind Aphid (Plants (Basel, Switzerland) · 2025Article
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Article
- ACL1-ROC4/5 complex reveals a common mechanism in rice response to brown planthopper infestation and drought.Nature communications · 2024Article
- Comparative transcriptome analysis reveals genes involved in trichome development and metabolism in tobacco.BMC plant biology · 2024Article
- Overexpression of the WRKY transcription factor gene NtWRKY65 enhances salt tolerance in tobacco (Nicotiana tabacum).BMC plant biology · 2024Article
- Transcriptome disclosure of hormones inducing stigma exsertion in Nicotiana tabacum by corolla shortening.BMC genomics · 2024Article
- A non-canonicalFrontiers in plant science · 2024Article
- Development of a MAGIC population and high-resolution quantitative trait mapping for nicotine content in tobacco.Frontiers in plant science · 2022Article
- Molecular Mechanisms of Plant Trichome Development.Frontiers in plant science · 2022Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
key messageNtCycB2 negatively regulates the initiation of tobacco long stalk glandular trichomes and influences the expression of diterpenoid biosynthesis- and environmental stress resistance-related genes. Many asterid plants possess multicellular trichomes on their surface, both glandular and non-glandular. The CycB2 gene plays a key role in multicellular trichome initiation, but has distinct effects on different types of trichomes; its mechanisms remain unknown. In tomato (Solanum lycopersicum), SlCycB2 negatively regulates non-glandular trichome formation, but its effects on glandular trichomes are ambiguous. In this study, we cloned the SlCycB2 homolog of Nicotiana tabacum, NtCycB2, and analyzed its effect on three types of trichomes, long stalk glandular trichomes (LGT), short stalk glandular trichomes (SGT), and non-glandular trichomes (NGT). Knocking out NtCycB2 (NtCycB2-KO) promoted LGT formation, while overexpression of NtCycB2 (NtCycB2-OE) decreased LGT density. SGT and NGT were not significantly influenced in either NtCycB2-KO or NtCycB2-OE plants, indicating that NtCycB2 regulated only LGT formation in tobacco. In addition, compared with NtCycB2-OE and control plants, NtCycB2-KO plants produced more trichome exudates, including diterpenoids and sugar esters, and exhibited stronger aphid resistance. To further elucidate the function of NtCycB2, RNA-Seq analysis of the NtCycB2-KO, NtCycB2-OE, and control plants was conducted. 2,552 and 1,933 differentially expressed genes (DEGs) were found in NtCycB2-KO and NtCycB2-OE plants, respectively. Gene Ontology analysis of the common DEGs revealed that ion transport, carbohydrate and amino acid metabolism, photosynthesis, and transcription regulation processes were significantly enriched. Among these DEGs, diterpenoid biosynthesis genes were upregulated in NtCycB2-KO plants and downregulated in NtCycB2-OE plants. Two MYB transcription factors and several stress resistance-related genes were also identified, suggesting they may participate in regulating LGT formation and aphid resistance.
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