Evidence map›Paper›PMID 34825784›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2022

Single-Cell Transcriptome Profiling Reveals Multicellular Ecosystem of Nucleus Pulposus during Degeneration Progression.

Ji Tu, Wentian Li, Sidong Yang, Pengyi Yang, Qi Yan, Shenyu Wang, Kaitao Lai, Xupeng Bai, Cenhao Wu, Wenyuan Ding and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
110citing papers in PubMed, 1 pooled it
11.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

110 citing papers in PubMed, 1 synthesis or guideline pooled it, 120 citations in OpenAlex.

  1. Pooled it
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  4. ACSL4-Dependent Lysosomal Lipid Peroxidation Links WTAP-Mediated mAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
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50 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 6 institutions in 2 countries.

Ji TuDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.ORCID 0000-0001-7933-3016
Wentian LiSpine Labs, St. George and Sutherland Clinical School, Faculty of Medicine, University of New South Wales, Sydney, New South Wales, 2217, Australia.ORCID 0000-0002-1474-5703
Sidong YangAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Brisbane, Queensland, 4072, Australia.
Pengyi YangCharles Perkins Centre, The University of Sydney, Sydney, NSW, 2006, Australia.
Qi YanDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.
Shenyu WangDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.
Kaitao LaiThe ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, NSW, 2139, Australia.
Xupeng BaiCancer Care Centre, St. George and Sutherland Clinical School, Faculty of Medicine, University of New South Wales, Sydney, New South Wales, 2052, Australia.
Cenhao WuDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.
Wenyuan DingDepartment of Spine Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, 05000, China.
Justin Cooper-WhiteAustralian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, Brisbane, Queensland, 4072, Australia.ORCID 0000-0002-1920-8229
Ashish DiwanSpine Labs, St. George and Sutherland Clinical School, Faculty of Medicine, University of New South Wales, Sydney, New South Wales, 2217, Australia.ORCID 0000-0003-1037-8421
Cao YangDepartment of Orthopaedic Surgery, Wuhan Union Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, Hubei, 430022, China.
Huilin YangDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.
Jun ZouDepartment of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.ORCID 0000-0003-0443-3156
Soochow University · CNSutherland Hospital · AUHebei Medical University · CNThe University of Sydney · AUThe University of Queensland · AUWuhan Union Hospital · CN

Funding

China Scholarship Council-UNSW ScholarshipNational Natural Science Foundation of China 82030068National Natural Science Foundation of China 82172506National Natural Science Foundation of China 91849114University International Postgraduate Award of University of New South Wales
6 · The paper itself

Abstract

Although degeneration of the nucleus pulposus (NP) is a major contributor to intervertebral disc degeneration (IVDD) and low back pain, the underlying molecular complexity and cellular heterogeneity remain poorly understood. Here, a comprehensive single-cell resolution transcript landscape of human NP is reported. Six novel human NP cells (NPCs) populations are identified by their distinct molecular signatures. The potential functional differences among NPC subpopulations are analyzed. Predictive transcripts, transcriptional factors, and signal pathways with respect to degeneration grades are explored. It is reported that fibroNPCs is the subpopulation for end-stage degeneration. CD90+NPCs are observed to be progenitor cells in degenerative NP tissues. NP-infiltrating immune cells comprise a previously unrecognized diversity of cell types, including granulocytic myeloid-derived suppressor cells (G-MDSCs). Integrin αM (CD11b) and oxidized low density lipoprotein receptor 1 (OLR1) as surface markers of NP-derived G-MDSCs are uncovered. The G-MDSCs are found to be enriched in mildly degenerated (grade II and III) NP tissues compared to severely degenerated (grade IV and V) NP tissues. Their immunosuppressive function and alleviation effects on NPCs' matrix degradation are revealed in vitro. Collectively, this study reveals the NPC-type complexity and phenotypic characteristics in NP, thereby providing new insights and clues for IVDD treatment.

Indexed as

FemaleGene Expression ProfilingHumansIntervertebral DiscIntervertebral Disc DegenerationMaleMiddle AgedNucleus PulposusSignal TransductionStem Cellsintervertebral disc degenerationlow back painnucleus pulposussingle-cell RNA sequencing

Identifiers

PMID34825784
PMCPMC8787427
OpenAlexW3215127319

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.