Evidence map›Paper›PMID 34825212›Full record

ArticleSTAR protocols2021

Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers.

Nicole Amberg, Simon Hippenmeyer

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Nicole AmbergInstitute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria.
Simon HippenmeyerInstitute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria.
Institute of Science and Technology Austria · AT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis. For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021).

Indexed as

Genetic TechniquesMosaicismAnimalsCell LineageFemaleGenetic MarkersHistocytochemistryImage Processing, Computer-AssistedMaleMiceMicroscopy, ConfocalSingle-Cell AnalysisGenetic MarkersDevelopmental biologyGeneticsMicroscopyModel OrganismsMolecular BiologyNeuroscienceSingle CellStem CellsTissue Engineering

Identifiers

PMID34825212
PMCPMC8603308
OpenAlexW3211886838

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.