ArticleEcology and evolution2021
Comparing whole-genome shotgun sequencing and DNA metabarcoding approaches for species identification and quantification of pollen species mixtures.
Article in Ecology and evolution, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Comparing Accuracy and Biases of DNA Metabarcoding, Hybridization Capture, and Metagenomic Sequencing for Quantifying Herbivore Diets.Molecular ecology resources · 2026Article
- Long-read nanopore shotgun metagenomic DNA sequencing for river biodiversity, wildlife, pollution, and environmental health monitoring.NAR genomics and bioinformatics · 2026Article
- Genomic insights into a versatile deep-sea methanotroph constituting the rare biosphere of a Brazilian carbonate mound complex.mSystems · 2026Article
- Metagenomics reveals contrasted responses of microbial communities to wheat straw amendment in cropland and grassland soils.Scientific reports · 2025Article
- A Novel Approach for Pollen Identification and Quantification Using Hybrid Capture-Based DNA Metabarcoding.Ecology and evolution · 2025Article
- Exploring microbial diversity in the rhizosphere: a comprehensive review of metagenomic approaches and their applications.3 Biotech · 2024Review
- Semi-automated sequence curation for reliable reference datasets in ITS2 vascular plant DNA (meta-)barcoding.Scientific data · 2024Article
- Article
- Diverse patterns of correspondence between protist metabarcodes and protist metagenome-assembled genomes.PloS one · 2024Article
- Plants, pollinators and their interactions under global ecological change: The role of pollen DNA metabarcoding.Molecular ecology · 2023Article
- Honey Quality Control: Review of Methodologies for Determining Entomological Origin.Molecules (Basel, Switzerland) · 2023Review
- Investigating the use of pollen DNA metabarcoding to quantify bee foraging and effects of threshold selection.PloS one · 2023Article
- Characterizing a subtropical hypereutrophic lake: From physicochemical variables to shotgun metagenomic data.Frontiers in microbiology · 2022Article
- Comparing whole-genome shotgun sequencing and DNA metabarcoding approaches for species identification and quantification of pollen species mixtures.Ecology and evolution · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular identification of mixed-species pollen samples has a range of applications in various fields of research. To date, such molecular identification has primarily been carried out via amplicon sequencing, but whole-genome shotgun (WGS) sequencing of pollen DNA has potential advantages, including (1) more genetic information per sample and (2) the potential for better quantitative matching. In this study, we tested the performance of WGS sequencing methodology and publicly available reference sequences in identifying species and quantifying their relative abundance in pollen mock communities. Using mock communities previously analyzed with DNA metabarcoding, we sequenced approximately 200Mbp for each sample using Illumina HiSeq and MiSeq. Taxonomic identifications were based on the Kraken
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