ArticleNature methods2021
Anterograde transneuronal tracing and genetic control with engineered yellow fever vaccine YFV-17D.
Article in Nature methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- WTR: A Toolkit for Functional Anterograde Transsynaptic Circuit Mapping.Research square · 2026Article
- TRACR: an anterograde transneuronal tracing system for genetic access across synapses and longitudinal circuit analysis.bioRxiv : the preprint server for biology · 2026Article
- Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas.Nature protocols · 2026Review
- HSV-1 strain H129 co-opts neuronal synaptic transmission machinery for its transsynaptic spread.Nature neuroscience · 2026Article
- Recent optical approaches for anatomical and functional dissection of neuron-astrocyte circuitry.The Journal of physiology · 2026Review
- Preparation and application of YFV-17D-derived Anterograde Trans-neuronal Viral Vectors for Neuroscience Research.bioRxiv : the preprint server for biology · 2026Article
- Hippocampal neural circuit mechanisms in Alzheimer's disease revealed by viral-genetic circuit mapping.Neurobiology of disease · 2025Review
- Article
- Tracing neural circuits in the anterograde direction.Nature methods · 2025Article
- ATLAS: a rationally designed anterograde transsynaptic tracer.Nature methods · 2025Article
- Suppressing maize stalk rot through promoted rhizosphere microbial recruitment in cultivar mixtures.Frontiers in microbiology · 2025Article
- Antipsoriatic Effect of Silymarin NLCs Based Gel: In Vitro and In Vivo Activity.AAPS PharmSciTech · 2024Article
- Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing.eLife · 2024Article
- Immunolabeling-compatible PEGASOS tissue clearing for high-resolution whole mouse brain imaging.Frontiers in neural circuits · 2024Article
- Brain-wide circuit-specific targeting of astrocytes.Cell reports methods · 2023Article
- Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA andbioRxiv : the preprint server for biology · 2023Article
- Probing neural circuit mechanisms in Alzheimer's disease using novel technologies.Molecular psychiatry · 2023Review
- A rabies virus-based toolkit for efficient retrograde labeling and monosynaptic tracing.Neural regeneration research · 2023Article
- Directed stepwise tracing of polysynaptic neuronal circuits with replication-deficient pseudorabies virus.Cell reports methods · 2023Article
- Monosynaptic restriction of the anterograde herpes simplex virus strain H129 for neural circuit tracing.The Journal of comparative neurology · 2023Article
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Authors and funding
15 authors at 3 institutions in 1 country.
Funding
Abstract
Transneuronal viruses are powerful tools for tracing neuronal circuits or delivering genes to specific neurons in the brain. While there are multiple retrograde viruses, few anterograde viruses are available. Further, available anterograde viruses often have limitations such as retrograde transport, high neuronal toxicity or weak signals. We developed an anterograde viral system based on a live attenuated vaccine for yellow fever-YFV-17D. Replication- or packaging-deficient mutants of YFV-17D can be reconstituted in the brain, leading to efficient synapse-specific and anterograde-only transneuronal spreading, which can be controlled to achieve either monosynaptic or polysynaptic tracing. Moreover, inducible transient replication of YFV-17D mutant is sufficient to induce permanent transneuronal genetic modifications without causing neuronal toxicity. The engineered YFV-17D systems can be used to express fluorescent markers, sensors or effectors in downstream neurons, thus providing versatile tools for mapping and functionally controlling neuronal circuits.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.