ArticleBMC research notes2021
A new primer pair for barcoding of bees (Hymenoptera: Anthophila) without amplifying the orthologous coxA gene of Wolbachia bacteria.
Article in BMC research notes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 33 citations in OpenAlex.
- Identification of Ctenocephalides felis felis and the detection and characterization of associated Wolbachia endosymbiont in Pakistan.BMC veterinary research · 2026Article
- What lurks in the dark? An innovative framework for studying diverse wild insect microbiota.Microbiome · 2025Article
- Complete Mitochondrial Genome Characterization and Phylogenomics of the Stingless Bee,Insects · 2025Article
- Barcoding the Caatinga biome bees: a practical review.Molecular biology reports · 2025Review
- Comparing modern identification methods for wild bees: Metabarcoding and image-based morphological taxonomic assignment.PloS one · 2024Article
- New species based on the biological species concept within the complex ofEcology and evolution · 2023Article
- A novel and non-invasive method for DNA extraction from dry bee specimens.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesDNA barcoding became an effective method for the identification and monitoring of bees. However, standard primer pairs used for barcoding often result in (co-) amplification of bacterial endosymbionts of the genus Wolbachia, which are widespread among bee species. Here we designed a new primer pair and compared it with the performance of the standard Folmer-primers for a small sample set of bees representing the main taxonomic groups of bees.
resultsThe newly designed primer pair (BeeCox1F1/BeeCox1R2) outperformed the standard barcoding primer (LCO1490/HCO2198). By generating barcodes for a small test set of bees we found that the new primer pair produced high-quality sequences in all cases for unambiguous species identification using BOLD. Conversely, the standard barcoding primers often co-amplified the homologous Wolbachia gene and resulted in mixed chromatogram signals. These sequences showed high similarity with the bacterial endosymbiont instead of the host.
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