ArticleCartilage2021
Methodology to Quantify Collagen Subtypes and Crosslinks: Application in Minipig Cartilages.
Article in Cartilage, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Isolation, purification and characterization of lipocartilage in mice.Nature protocols · 2026Review
- Innovative Approaches in Microtia Treatment: Advancements in Tissue Engineering and Scaffold Design.Annals of biomedical engineering · 2025Review
- Characterization of the Age-Related Differences in Porcine Acetabulum and Femoral Head Articular Cartilage.Cartilage · 2025Article
- Omics in mini-livestock: a genomic perspective on the future of sustainable food systems.Frontiers in genetics · 2025Review
- A cell bank paradigm for preclinical evaluation of an analogous cellular product for an allogeneic cell therapy.Biofabrication · 2024Article
- The Longer-Term Effects of a Single Bupivacaine Exposure on the Mechanical Properties of Native Cartilage Explants.Cartilage · 2024Article
- Electrical Stimulation of Mesenchymal Stem Cells as a Tool for Proliferation and Differentiation in Cartilage Tissue Engineering: A Scaffold-Based Approach.Bioengineering (Basel, Switzerland) · 2024Article
- Tissue anisotropy and collagenomics in porcine penile tunica albuginea: Implications for penile structure-function relationships and tissue engineering.Acta biomaterialia · 2023Article
- Article
- Characterization of the Temporomandibular Joint Disc Complex in the Yucatan Minipig.Tissue engineering. Part A · 2023Article
- Ion modulatory treatments toward functional self-assembled neocartilage.Acta biomaterialia · 2022Article
- Navigating regulatory pathways for translation of biologic cartilage repair products.Science translational medicine · 2022Review
- Proteomic, mechanical, and biochemical development of tissue-engineered neocartilage.Biomaterials research · 2022Article
- Cell mediated remodeling of stiffness matched collagen and fibrin scaffolds.Scientific reports · 2022Article
- Proteomic, mechanical, and biochemical characterization of cartilage development.Acta biomaterialia · 2022Article
- Yucatan Minipig Knee Meniscus Regional Biomechanics and Biochemical Structure Support its Suitability as a Large Animal Model for Translational Research.Frontiers in bioengineering and biotechnology · 2022Article
- Therapeutic effect of alternating red and blue light irradiation combined with collagen in patients with acne vulgaris and the risk factors of short-term recurrence.American journal of translational research · 2022Article
- Cross-linking methods of type I collagen-based scaffolds for cartilage tissue engineering.American journal of translational research · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
introductionThis study develops assays to quantify collagen subtypes and crosslinks with liquid chromatography-mass spectrometry (LC-MS) and characterizes the cartilages in the Yucatan minipig.
methodsFor collagen subtyping, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed on tissues digested in trypsin. For collagen crosslinks, LC-MS analysis was performed on hydrolysates. Samples were also examined histologically and with bottom-up proteomics. Ten cartilages (femoral condyle, femoral head, facet joint, floating rib, true rib, auricular cartilage, annulus fibrosus, 2 meniscus locations, and temporomandibular joint disc) were analyzed.
resultsThe collagen subtyping assay quantified collagen types I and II. The collagen crosslinks assay quantified mature and immature crosslinks. Collagen subtyping revealed that collagen type I predominates in fibrocartilages and collagen type II in hyaline cartilages, as expected. Elastic cartilage and fibrocartilages had more mature collagen crosslink profiles than hyaline cartilages. Bottom-up proteomics revealed a spectrum of ratios between collagen types I and II, and quantified 42 proteins, including 24 collagen alpha-chains and 12 minor collagen types. DISCUSSION: The novel assays developed in this work are sensitive, inexpensive, and use a low operator time relative to other collagen analysis methods. Unlike the current collagen assays, these assays quantify collagen subtypes and crosslinks without an antibody-based approach or lengthy chromatography. They apply to any collagenous tissue, with broad applications in tissue characterization and tissue engineering. For example, a novel finding of this work was the presence of a large quantity of collagen type III in the white-white knee meniscus and a spectrum of hyaline and fibrous cartilages.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.