ArticleDevelopment (Cambridge, England)2021
A 37 kb region upstream of brachyury comprising a notochord enhancer is essential for notochord and tail development.
Article in Development (Cambridge, England), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- The notochord: development, disease and stem cell-based modelling.Development (Cambridge, England) · 2026Review
- A combination of transcriptomics and epigenomics identifies genes and regulatory elements involved in embryonic tail development in the mouse.BMC biology · 2025Article
- Timely TGFβ signalling inhibition induces notochord.Nature · 2025Article
- Article
- Genome-wide identification of notochord enhancers comprising the regulatory landscape of the brachyury locus in mouse.Development (Cambridge, England) · 2023Article
- Conserved enhancers control notochord expression of vertebrate Brachyury.Nature communications · 2023Article
- Development and Application of a High-Resolution Melting Analysis with Unlabeled Probes for the Screening of Short-Tailed SheepAnimals : an open access journal from MDPI · 2022Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The node-streak border region comprising notochord progenitor cells (NPCs) at the posterior node and neuro-mesodermal progenitor cells (NMPs) in the adjacent epiblast is the prime organizing center for axial elongation in mouse embryos. The T-box transcription factor brachyury (T) is essential for both formation of the notochord and maintenance of NMPs, and thus is a key regulator of trunk and tail development. The T promoter controlling T expression in NMPs and nascent mesoderm has been characterized in detail; however, control elements for T expression in the notochord have not been identified yet. We have generated a series of deletion alleles by CRISPR/Cas9 genome editing in mESCs, and analyzed their effects in mutant mouse embryos. We identified a 37 kb region upstream of T that is essential for notochord function and tailbud outgrowth. Within that region, we discovered a T-binding enhancer required for notochord cell specification and differentiation. Our data reveal a complex regulatory landscape controlling cell type-specific expression and function of T in NMP/nascent mesoderm and node/notochord, allowing proper trunk and tail development.
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