ArticleEnvironmental science and pollution research international2022
Inter-laboratory validation of an ISO test method for measuring enzyme activities in soil samples using colorimetric substrates.
Article in Environmental science and pollution research international, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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Who cites it
3 citing papers in PubMed.
- Interlaboratory validation of an optimized protocol for measuring α-amylase activity by the INFOGEST international research network.Scientific reports · 2025Article
- Article
- RISMEAU dataset: Pharmaceuticals and biocides concentrations in urban and agricultural sludge, amended soil and leachate and their environmental impacts.Data in brief · 2024Article
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Authors and funding
15 authors.
Funding
Abstract
The evaluation of soil quality requires the use of robust methods to assess biologically based indicators. Among them, enzyme activities are used for several decades, but there is a clear need to update their measurement methods for routine use, in combining feasibility, accuracy, and reliability. To this end, the platform Biochem-Env optimized a miniaturized method to measure enzyme activities in soils using colorimetric substrates in micro-well plates. The standardization of the method was carried out within the framework of ISO/TC 190/SC 4/WG 4 "Soil quality - Biological methods" workgroup, recommending an inter-laboratory evaluation for the publication of a full ISO standard. That evaluation, managed by the platform, was based on the measurement, in six soils of contrasted physicochemical properties, of the ten soil enzyme activities described in the standard. Eight laboratories were involved in the validation study. Only 2.7% of outliers were identified from the analyses of the whole dataset. The repeatability and reproducibility of the method were determined by computing, respectively, the intra-laboratory (CV
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