ArticleBiosensors2021
High-Throughput, Label-Free Isolation of White Blood Cells from Whole Blood Using Parallel Spiral Microchannels with U-Shaped Cross-Section.
Article in Biosensors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- A Consecutive Separation Strategy Using a 3D-Printed Microfluidic Chip to Achieve High-Purity White Blood Cells From Blood.Electrophoresis · 2026Article
- A Cascaded Dual Spiral Microfluidic Chip for Continuous Separation of Multicomponent Microparticles.Micromachines · 2026Article
- Dean migration mechanisms: facilitating the control of microbial contaminants in cell therapy product manufacturing.Microsystems & nanoengineering · 2026Article
- Effects of Shear and Extensional Stresses on Cells: Investigation in a Spiral Microchannel and Contraction-Expansion Arrays.ACS biomaterials science & engineering · 2025Review
- High-efficient white blood cell separation from whole blood using cascaded inertial microfluidics.Talanta · 2025Article
- Recent developments in microfluidic passive separation to enable purification of platelets for transfusion.Biomicrofluidics · 2024Review
- Characterization of the phagocytic ability of white blood cells separated using a single curvature spiral microfluidic device.Biomedical engineering letters · 2024Article
- Simultaneous high-throughput particle-bacteria separation and solution exchange via in-plane and out-of-plane parallelization of microfluidic centrifuges.Biomicrofluidics · 2024Article
- Micropump integrated white blood cell separation platform for detection of chronic granulomatous disease.Mikrochimica acta · 2024Article
- Separation of microalgae from bacterial contaminants using spiral microchannel in the presence of a chemoattractant.Bioresources and bioprocessing · 2024Article
- Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit.Micromachines · 2023Review
- Fabrication of Spiral Low-Cost Microchannel with Trapezoidal Cross Section for Cell Separation Using a Grayscale Approach.Micromachines · 2023Article
- Microfluidics for Biomedical Applications.Biosensors · 2023Article
- A dielectrophoresis-based microfluidic system having double-sided optimized 3D electrodes for label-free cancer cell separation with preserving cell viability.Scientific reports · 2022Article
- Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid isolation of white blood cells (WBCs) from whole blood is an essential part of any WBC examination platform. However, most conventional cell separation techniques are labor-intensive and low throughput, require large volumes of samples, need extensive cell manipulation, and have low purity. To address these challenges, we report the design and fabrication of a passive, label-free microfluidic device with a unique U-shaped cross-section to separate WBCs from whole blood using hydrodynamic forces that exist in a microchannel with curvilinear geometry. It is shown that the spiral microchannel with a U-shaped cross-section concentrates larger blood cells (e.g., WBCs) in the inner cross-section of the microchannel by moving smaller blood cells (e.g., RBCs and platelets) to the outer microchannel section and preventing them from returning to the inner microchannel section. Therefore, it overcomes the major limitation of a rectangular cross-section where secondary Dean vortices constantly enforce particles throughout the entire cross-section and decrease its isolation efficiency. Under optimal settings, we managed to isolate more than 95% of WBCs from whole blood under high-throughput (6 mL/min), high-purity (88%), and high-capacity (360 mL of sample in 1 h) conditions. High efficiency, fast processing time, and non-invasive WBC isolation from large blood samples without centrifugation, RBC lysis, cell biomarkers, and chemical pre-treatments make this method an ideal choice for downstream cell study platforms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.