Evidence map›Paper›PMID 34820909›Full record

Trial reportFASEB journal : official publication of the Federation of American Societies for Experimental Biology2021

Choline metabolome response to prenatal choline supplementation across pregnancy: A randomized controlled trial.

Siraphat Taesuwan, Melissa Q McDougall, Olga V Malysheva, Erica Bender, Julie E H Nevins, Srisatish Devapatla, Ramesh Vidavalur, Marie A Caudill, Kevin C Klatt

Erratum issuedOpen access · greenAbstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.

  1. Pooled it
  2. Trial
  3. Trial
  4. Article
  5. Review
  6. Review
  7. Review
  8. Choline-An Essential Nutrient with Health Benefits and a Signaling Molecule.International journal of molecular sciences · 2025
    Review
  9. Article
  10. Article
  11. Article
  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Choline supplements: An update.Frontiers in endocrinology · 2023
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Siraphat TaesuwanDivision of Nutritional Sciences, Cornell University, Ithaca, New York, USA.
Melissa Q McDougallDivision of Nutritional Sciences, Cornell University, Ithaca, New York, USA.
Olga V MalyshevaDivision of Nutritional Sciences, Cornell University, Ithaca, New York, USA.
Erica BenderDivision of Nutritional Sciences, Cornell University, Ithaca, New York, USA.
Julie E H NevinsDivision of Nutritional Sciences, Cornell University, Ithaca, New York, USA.
Srisatish DevapatlaCayuga Medical Center, Ithaca, New York, USA.
Ramesh VidavalurCayuga Medical Center, Ithaca, New York, USA.
Marie A CaudillDivision of Nutritional Sciences, Cornell University, Ithaca, New York, USA.
Kevin C KlattDivision of Nutritional Sciences, Cornell University, Ithaca, New York, USA.ORCID 0000-0003-0223-8250
Cornell University · US

Funding

TRAINING IN MATERNAL AND CHILD NUTRITIONT32HD087137 · NICHD · CORNELL UNIVERSITY · PI Laura Bellows, Julia L. Finkelstein · 2016 to 2026
$2.0M
NICHD NIH HHS T32 HD087137
6 · The paper itself

Abstract

Pregnancy places a unique stress upon choline metabolism, requiring adaptations to support both maternal and fetal requirements. The impact of pregnancy and prenatal choline supplementation on choline and its metabolome in free-living, healthy adults is relatively uncharacterized. This study investigated the effect of prenatal choline supplementation on maternal and fetal biomarkers of choline metabolism among free-living pregnant persons consuming self-selected diets. Participants were randomized to supplemental choline (as choline chloride) intakes of 550 mg/d (500 mg/d d0-choline + 50 mg/d methyl-d9-choline; intervention) or 25 mg/d d9-choline (control) from gestational week (GW) 12-16 until Delivery. Fasting blood and 24-h urine samples were obtained at study Visit 1 (GW 12-16), Visit 2 (GW 20-24), and Visit 3 (GW 28-32). At Delivery, maternal and cord blood and placental tissue samples were collected. Participants randomized to 550 (vs. 25) mg supplemental choline/d achieved higher (p < .05) plasma concentrations of free choline, betaine, dimethylglycine, phosphatidylcholine (PC), and sphingomyelin at one or more study timepoint. Betaine was most responsive to prenatal choline supplementation with increases (p ≤ .001) in maternal plasma observed at Visit 2-Delivery (relative to Visit 1 and control), as well as in the placenta and cord plasma. Notably, greater plasma enrichments of d3-PC and LDL-C were observed in the intervention (vs. control) group, indicating enhanced PC synthesis through the de novo phosphatidylethanolamine N-methyltransferase pathway and lipid export. Overall, these data show that prenatal choline supplementation profoundly alters the choline metabolome, supporting pregnancy-related metabolic adaptations and revealing biomarkers for use in nutritional assessment and monitoring during pregnancy.

Indexed as

Adaptation, PhysiologicalDietary SupplementsMetabolomeAdultCase-Control StudiesCholineFemaleFetal BloodFetusHumansPlacentaPregnancyYoung AdultCholinecholine metabolomePEMT pathwayphosphatidylcholinepregnancyprenatal choline supplementation

Identifiers

PMID34820909
PMCPMC10911820
OpenAlexW3215118323

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.