ArticleSTAR protocols2021
Protocol for analysis of mouse neutrophil NETosis by flow cytometry.
Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Experimental procedures for flow cytometry of wild-type mouse brain: a systematic review.Frontiers in immunology · 2023Pooled it
- Pegtarazimod limits acute graft-versus-host disease mortality and severity in mice and is tolerated in patients.The Journal of clinical investigation · 2026Article
- Neutrophils in cancer.Nature reviews. Cancer · 2026Review
- Spectral Flow Cytometry Method for Immunophenotyping Neutrophil Activation and NETs in an Acute Dust Exposure Model.Immunity, inflammation and disease · 2026Article
- Neutrophil Inflammasome Activation and Pyroptosis Induced by a Metalloproteinase via HIF-1α and Gasdermin D Pathways.Journal of innate immunity · 2026Article
- Prenatal exposure to Zika virus shapes offspring neutrophil function in a sex-specific manner.Nature communications · 2025Article
- Immunoreceptor CD300a regulates ischemic tissue damage and adverse remodeling in the mouse heart and kidney.The Journal of clinical investigation · 2025Article
- SUMOylation Regulates Neutrophil Phagocytosis and Migration.Pharmaceuticals (Basel, Switzerland) · 2025Article
- IL-6R Signaling Is Associated with PAD4 and Neutrophil Extracellular Trap Formation in Patients with STEMI.International journal of molecular sciences · 2025Article
- Dynamic STING repression orchestrates immune cell development and function.Science immunology · 2025Article
- Article
- Deficient neutrophil responses early in influenza infection promote viral replication and pulmonary inflammation.PLoS pathogens · 2025Article
- Glioblastoma induces the recruitment and differentiation of dendritic-like "hybrid" neutrophils from skull bone marrow.Cancer cell · 2024Article
- Microglial heterogeneity in the ischemic stroke mouse brain of both sexes.Genome medicine · 2024Article
- Isolating Immune Cells from Mouse Brain and Skull.Journal of visualized experiments : JoVE · 2024Article
- Neutrophils and Neutrophil Extracellular Traps Cause Vascular Occlusion and Delayed Cerebral Ischemia After Subarachnoid Hemorrhage in Mice.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- A Comparative Study of Different Protocols for Isolation of Murine Neutrophils from Bone Marrow and Spleen.International journal of molecular sciences · 2023Article
- The quorum-sensing peptidic inhibitor rescues host immune system eradication: A novel infectivity mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- ATG5 provides host protection acting as a switch in the atg8ylation cascade between autophagy and secretion.Developmental cell · 2023Article
- Single-cell RNA-seq of primary bone marrow neutrophils from female and male adult mice.Scientific data · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Studies involving neutrophils are steadily increasing, thus creating a need for more optimized and thorough protocols for studying neutrophil function. Here, we present our protocol for extracting mouse bone marrow neutrophils, estimating the purity of isolated neutrophils, and assessing their ability to induce NETosis upon an external cue. We test two isolation protocols that can be used to attain neutrophils to assess NETosis induction. This approach allows for the parallel assessment of NETosis induction in cohorts larger than 10 samples. For complete details on the use and execution of this protocol, please refer to Lu et al., 2021.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.