ArticleBioactive materials2022
Increased connectivity of hiPSC-derived neural networks in multiphase granular hydrogel scaffolds.
Article in Bioactive materials, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Adipose-mimetic granular hydrogels uncover biophysical cues driving breast cancer invasion.Cell biomaterials · 2026Article
- Gaseous liquid nitrogen-assisted cryo-printing strategies for challenging volumetric anisotropic tissues fabrication.Science advances · 2026Article
- Granular Hydrogels as Modular Biomaterials: From Structural Design to Biological Responses.Advanced healthcare materials · 2026Review
- Adipose-mimetic granular hydrogels uncover biophysical cues driving breast cancer invasion.bioRxiv : the preprint server for biology · 2025Article
- Practical Guide to the Design of Granular Hydrogels for Customizing Complex Cellular Microenvironments.Advanced healthcare materials · 2025Review
- Tissue-Engineered Three-Dimensional Platforms for Disease Modeling and Therapeutic Development.Tissue engineering. Part B, Reviews · 2025Review
- Automated cell properties toolbox from 3D bioprinted hydrogel scaffolds via deep learning and optical coherence tomography.Biomedical optics express · 2025Article
- Advances in biomaterial-based tissue engineering for peripheral nerve injury repair.Bioactive materials · 2025Review
- Biomaterials for neuroengineering: applications and challenges.Regenerative biomaterials · 2025Review
- Multiphoton imaging for quantification of mesenchymal stem cell survival and distribution in PEG granular hydrogel scaffolds post-implantation into rat cranial bone defects.Biomedical engineering advances · 2024Article
- Granular Hydrogels for Harnessing the Immune Response.Advanced healthcare materials · 2024Review
- The Evolution of Technology-Driven In Vitro Models for Neurodegenerative Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Emerging granular hydrogel bioinks to improve biological function in bioprinted constructs.Trends in biotechnology · 2024Review
- Biofabrication and biomanufacturing in Ireland and the UK.Bio-design and manufacturing · 2024Review
- 3D printed scaffolds based on hyaluronic acid bioinks for tissue engineering: a review.Biomaterials research · 2023Review
- The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration.International journal of molecular sciences · 2023Article
- Growth and differentiation of human induced pluripotent stem cell (hiPSC)-derived kidney organoids using fully synthetic peptide hydrogels.Bioactive materials · 2023Article
- A hyaluronic acid granular hydrogel nerve guidance conduit promotes regeneration and functional recovery of injured sciatic nerves in rats.Neural regeneration research · 2023Article
- Hydrogel Based on Nanoclay and Gelatin Methacrylate Polymeric Matrix as a Potential Osteogenic Application.Journal of functional biomaterials · 2023Article
- 3D Bioprinting of Induced Pluripotent Stem Cells and Disease Modeling.Handbook of experimental pharmacology · 2023Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To reflect human development, it is critical to create a substrate that can support long-term cell survival, differentiation, and maturation. Hydrogels are promising materials for 3D cultures. However, a bulk structure consisting of dense polymer networks often leads to suboptimal microenvironments that impedes nutrient exchange and cell-to-cell interaction. Herein, granular hydrogel-based scaffolds were used to support 3D human induced pluripotent stem cell (hiPSC)-derived neural networks. A custom designed 3D printed toolset was developed to extrude hyaluronic acid hydrogel through a porous nylon fabric to generate hydrogel granules. Cells and hydrogel granules were combined using a weaker secondary gelation step, forming self-supporting cell laden scaffolds. At three and seven days, granular scaffolds supported higher cell viability compared to bulk hydrogels, whereas granular scaffolds supported more neurite bearing cells and longer neurite extensions (65.52 ± 11.59 μm) after seven days compared to bulk hydrogels (22.90 ± 4.70 μm). Long-term (three-month) cultures of clinically relevant hiPSC-derived neural cells in granular hydrogels supported well established neuronal and astrocytic colonies and a high level of neurite extension both inside and beyond the scaffold. This approach is significant as it provides a simple, rapid and efficient way to achieve a tissue-relevant granular structure within hydrogel cultures.
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