Data on the Relationships of Signal-To-Cutoff Ratios of Elecsys HIV Antigen/Antibody and Elecsys Syphilis Assays to Subsequent Confirmatory Testing at an Academic Medical Center.

Immunoassays are widely used as screening tests for HIV and syphilis in clinical, public health, and research settings. For syphilis, immunoassays are used in 'reverse syphilis algorithms' that start with treponemal tests such as syphilis IgG or syphilis total antibodies for the initial screen. Many screening immunoassays for HIV and syphilis use signal-to-cutoff (S/CO) values for determining positivity, with a cutoff value such as 1.0 differentiating positive from negative. Published literature indicates that the S/CO value often correlates with the likelihood of subsequent confirmation of HIV and syphilis infections, with low S/CO values barely exceeding the cutoff more likely to represent false positive screens. The data in this article present results from the Roche Diagnostics Elecsys HIV combi PT Assay and the Elecsys Syphilis Assay. The data include 19,368 syphilis total antibody screening results on 15,764 unique patients and 28,952 HIV screening results on 24,556 unique patients, S/CO values, clinical area where testing was ordered, sex, and age. For samples with positive syphilis total antibody screens, the data also include results of RPR (the immediate next step in the reverse algorithm),