Evidence map›Paper›PMID 34820092›Full record

ArticleChemical science2021

Whole-cell screening of oxidative enzymes using genetically encoded sensors.

Tsvetan Kardashliev, Alexandra Weingartner, Elvira Romero, Ulrich Schwaneberg, Marco Fraaije, Sven Panke, Martin Held

Open access · diamondAbstract read
In one paragraph

Article in Chemical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

  1. Genuine Directed Evolution In Test Tube (GENie).bioRxiv : the preprint server for biology · 2026
    Article
  2. Activity assays for flavoprotein oxidases: an overview.Applied microbiology and biotechnology · 2025
    Review
  3. Article
  4. ACS omega · 2023
    Review
  5. Leveraging intrinsic flexibility to engineer enhanced enzyme catalytic activity.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Tsvetan KardashlievDepartment of Biosystems Science and Engineering ETH Zurich, Mattenstrasse 26 4058 Basel Switzerland heldma@ethz.ch tsvetank@ethz.ch.ORCID https://orcid.org/0000-0001-8328-4616
Alexandra WeingartnerInstitute of Biotechnology, RWTH Aachen University Worringerweg 3 52074 Aachen Germany.
Elvira RomeroFaculty of Science and Engineering, University of Groningen Nijenborgh 4 9747 AG Groningen The Netherlands.
Ulrich SchwanebergInstitute of Biotechnology, RWTH Aachen University Worringerweg 3 52074 Aachen Germany.ORCID https://orcid.org/0000-0003-4026-701X
Marco FraaijeFaculty of Science and Engineering, University of Groningen Nijenborgh 4 9747 AG Groningen The Netherlands.ORCID https://orcid.org/0000-0001-6346-5014
Sven PankeDepartment of Biosystems Science and Engineering ETH Zurich, Mattenstrasse 26 4058 Basel Switzerland heldma@ethz.ch tsvetank@ethz.ch.
Martin HeldDepartment of Biosystems Science and Engineering ETH Zurich, Mattenstrasse 26 4058 Basel Switzerland heldma@ethz.ch tsvetank@ethz.ch.ORCID https://orcid.org/0000-0002-5952-5659
ETH Zurich · CHRWTH Aachen University · DEUniversity of Groningen · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biocatalysis is increasingly used for synthetic purposes in the chemical and especially the pharmaceutical industry. Enzyme discovery and optimization which is frequently needed to improve biocatalytic performance rely on high-throughput methods for activity determination. These methods should ideally be generic and applicable to entire enzyme families. Hydrogen peroxide (H

Identifiers

PMID34820092
PMCPMC8597865
OpenAlexW3209668395

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.