ArticleProceedings of the National Academy of Sciences of the United States of America2021
Three-dimensional virtual histology of the human hippocampus based on phase-contrast computed tomography.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- X-ray histology on rapidly fixed fresh tumor tissue samples for fast resection margin assessment.Scientific reports · 2026Article
- Quantitative Stain Mapping in X-Ray Virtual Histology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Three-dimensional high-content imaging of unstained soft tissue with subcellular resolution using a laboratory-based X-ray microscope.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- 3D imaging of neuronal inclusions and protein aggregates in human neurodegeneration by multiscale x-ray phase-contrast tomography.Brain pathology (Zurich, Switzerland) · 2026Article
- X-ray waveguide optics at GINIX/P10 PETRA III: recent progress and future directions.Journal of synchrotron radiation · 2026Article
- The Extremely Brilliant Brain: An Isotropic Microscale Human Brain Dataset.bioRxiv : the preprint server for biology · 2026Article
- Fractal Dimension in Healthy Hippocampi and Hippocampi With Mesial Temporal Sclerosis: An Observational Study.Cureus · 2025Article
- Mass spectrometric imaging of organic and metallic metabolites by plasmon-induced interfacial charge-transfer transition (PICTT) on Au sputtered ITO slides.Chemical science · 2025Article
- 3D virtual histology of rodent and primate cochleae with multi-scale phase-contrast X-ray tomography.Scientific reports · 2025Article
- Synchrotron X-ray imaging of soft biological tissues - principles, applications and future prospects.Journal of cell science · 2024Review
- Volumetric changes of the enteric nervous system under physiological and pathological conditions measured using x-ray phase-contrast tomography.JGH open : an open access journal of gastroenterology and hepatology · 2024Article
- Automated deep learning segmentation of high-resolution 7 Tesla postmortem MRI for quantitative analysis of structure-pathology correlations in neurodegenerative diseases.Imaging neuroscience (Cambridge, Mass.) · 2024Article
- Revealing complexity: segmentation of hippocampal subfields in adolescents with major depressive disorder reveals specific links to cognitive dysfunctions.European psychiatry : the journal of the Association of European Psychiatrists · 2024Article
- Neodymium acetate as a contrast agent for X-ray phase-contrast tomography.Journal of medical imaging (Bellingham, Wash.) · 2023Article
- Fusome topology and inheritance during insect gametogenesis.PLoS computational biology · 2023Article
- DNA Damage Response-Associated Cell Cycle Re-Entry and Neuronal Senescence in Brain Aging and Alzheimer's Disease.Journal of Alzheimer's disease : JAD · 2023Review
- Pathological Nuclear Hallmarks in Dentate Granule Cells of Alzheimer's Patients: A Biphasic Regulation of Neurogenesis.International journal of molecular sciences · 2022Article
- An optimized workflow for microCT imaging of formalin-fixed and paraffin-embedded (FFPE) early equine embryos.Anatomia, histologia, embryologia · 2022Article
- Structure of the myenteric plexus in normal and diseased human ileum analyzed by X-ray virtual histology slices.World journal of gastroenterology · 2022Article
- The synchrotron radiation source PETRA III and its future ultra-low-emittance upgrade PETRA IV.European physical journal plus · 2022Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have studied the three-dimensional (3D) cytoarchitecture of the human hippocampus in neuropathologically healthy and Alzheimer's disease (AD) individuals, based on phase-contrast X-ray computed tomography of postmortem human tissue punch biopsies. In view of recent findings suggesting a nuclear origin of AD, we target in particular the nuclear structure of the dentate gyrus (DG) granule cells. Tissue samples of 20 individuals were scanned and evaluated using a highly automated approach of measurement and analysis, combining multiscale recordings, optimized phase retrieval, segmentation by machine learning, representation of structural properties in a feature space, and classification based on the theory of optimal transport. Accordingly, we find that the prototypical transformation between a structure representing healthy granule cells and the pathological state involves a decrease in the volume of granule cell nuclei, as well as an increase in the electron density and its spatial heterogeneity. The latter can be explained by a higher ratio of heterochromatin to euchromatin. Similarly, many other structural properties can be derived from the data, reflecting both the natural polydispersity of the hippocampal cytoarchitecture between different individuals in the physiological context and the structural effects associated with AD pathology.
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