Evidence map›Paper›PMID 34819371›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2021

Plant-environment microscopy tracks interactions of

Yangminghao Liu, Daniel Patko, Ilonka Engelhardt, Timothy S George, Nicola R Stanley-Wall, Vincent Ladmiral, Bruno Ameduri, Tim J Daniell, Nicola Holden, Michael P MacDonald and 1 more

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

  1. Review
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  3. Article
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  6. Article
  7. Optimizing Conditions forPlants (Basel, Switzerland) · 2025
    Article
  8. Article
  9. Dynamic in situ detection in iRhizo-Chip reveals diurnal fluctuations ofProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  10. Article
  11. Article
  12. Article
  13. Review
  14. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yangminghao LiuSchool of Science and Engineering, University of Dundee, Dundee DD1 4HN, United Kingdom.ORCID 0000-0003-3730-0464
Daniel PatkoEcological Sciences, The James Hutton Institute, Dundee DD2 5DA, United Kingdom.
Ilonka EngelhardtEcological Sciences, The James Hutton Institute, Dundee DD2 5DA, United Kingdom.ORCID 0000-0001-8280-4704
Timothy S GeorgeEcological Sciences, The James Hutton Institute, Dundee DD2 5DA, United Kingdom.
Nicola R Stanley-WallSchool of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom.ORCID 0000-0002-5936-9721
Vincent LadmiralInstitut Charles Gerhardt de Montpellier, Université de Montpellier, CNRS, ENSCM, Montpellier 34090, France.ORCID 0000-0002-7590-4800
Bruno AmeduriInstitut Charles Gerhardt de Montpellier, Université de Montpellier, CNRS, ENSCM, Montpellier 34090, France.
Tim J DaniellPlants, Photosynthesis and Soil, School of Biosciences, The University of Sheffield, Sheffield S10 2TN, United Kingdom.ORCID 0000-0003-0435-4343
Nicola HoldenNorthern Faculty, Scotland's Rural College, Aberdeen AB21 9YA, United Kingdom.ORCID 0000-0002-7904-4529
Michael P MacDonaldSchool of Science and Engineering, University of Dundee, Dundee DD1 4HN, United Kingdom; M.P.MacDonald@dundee.ac.uk ldupuy@neiker.eus.ORCID 0000-0003-4415-5437
Lionel X DupuyEcological Sciences, The James Hutton Institute, Dundee DD2 5DA, United Kingdom; M.P.MacDonald@dundee.ac.uk ldupuy@neiker.eus.ORCID 0000-0001-5221-9037

Funding

Biotechnology and Biological Sciences Research Council BB/P001335/1Biotechnology and Biological Sciences Research Council BB/R012415/1
6 · The paper itself

Abstract

Our understanding of plant-microbe interactions in soil is limited by the difficulty of observing processes at the microscopic scale throughout plants' large volume of influence. Here, we present the development of three-dimensional live microscopy for resolving plant-microbe interactions across the environment of an entire seedling growing in a transparent soil in tailor-made mesocosms, maintaining physical conditions for the culture of both plants and microorganisms. A tailor-made, dual-illumination light sheet system acquired photons scattered from the plant while fluorescence emissions were simultaneously captured from transparent soil particles and labeled microorganisms, allowing the generation of quantitative data on samples ∼3,600 mm

Indexed as

RhizosphereBacillus subtilisCalibrationEnvironmentEquipment DesignFluorescenceImage Processing, Computer-AssistedLactucaMicroscopyPlant RootsSeedlingsSiliconSoilSoil MicrobiologyTemperatureSiliconSoilenvironmental imagingrhizosphereroot–microbe interactions

Identifiers

PMID34819371
PMCPMC8640753

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.