ArticleVeterinary research2021
Novel chicken two-dimensional intestinal model comprising all key epithelial cell types and a mesenchymal sub-layer.
Article in Veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 30 citations in OpenAlex.
- Animal organoids as models for integrated One Health research.One health (Amsterdam, Netherlands) · 2026Review
- Advances in Poultry RNA-Omics Research: Technologies, RNA Information Layers, and Applications in Complex Traits.Animals : an open access journal from MDPI · 2026Review
- Novel organoids andParasitology · 2025Review
- Long-term culture of chicken tracheal organoids for the purpose of avian influenza virus research.Virology journal · 2025Article
- Morphological and immunological characterization of primary cultured chicken caecal epithelial cells.European journal of microbiology & immunology · 2024Article
- Discrimination of distinct chicken M cell subsets based on CSF1R expression.Scientific reports · 2024Article
- Chicken intestinal organoids: a novel method to measure the mode of action of feed additives.Frontiers in immunology · 2024Article
- 3D sheep rumen epithelial structures driven from single cells in vitro.Veterinary research · 2023Article
- Paneth cells in farm animals: current status and future direction.Journal of animal science and biotechnology · 2023Review
- Differential Salmonella Typhimurium intracellular replication and host cell responses in caecal and ileal organoids derived from chicken.Veterinary research · 2023Article
- Disentangling the innate immune responses of intestinal epithelial cells and lamina propria cells toFrontiers in microbiology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
The intestinal epithelium plays a variety of roles including providing an effective physical barrier and innate immune protection against infection. Two-dimensional models of the intestinal epithelium, 2D enteroids, are a valuable resource to investigate intestinal cell biology and innate immune functions and are suitable for high throughput studies of paracellular transport and epithelial integrity. We have developed a chicken 2D enteroid model that recapitulates all major differentiated cell lineages, including enterocytes, Paneth cells, Goblet cells, enteroendocrine cells and leukocytes, and self-organises into an epithelial and mesenchymal sub-layer. Functional studies demonstrated the 2D enteroids formed a tight cell layer with minimal paracellular flux and a robust epithelial integrity, which was maintained or rescued following damage. The 2D enteroids were also able to demonstrate appropriate innate immune responses following exposure to bacterial endotoxins, from Salmonella enterica serotype Typhimurium and Bacillus subtilis. Frozen 2D enteroids cells when thawed were comparable to freshly isolated cells. The chicken 2D enteroids provide a useful ex vivo model to study intestinal cell biology and innate immune function, and have potential uses in screening of nutritional supplements, pharmaceuticals, and bioactive compounds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.