Evidence map›Paper›PMID 34819030›Full record

ArticleBMC genomics2021

Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping.

Baowei Li, Yanran Liu, Xiaodan Hao, Jinhua Dong, Limei Chen, Haimei Li, Wei Wu, Ying Liu, Jianxun Wang, Yin Wang and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. An Assay for Variable Fragment Length Allele-Specific Genotyping.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Baowei LiKey Laboratory of Biological Medicines in Universities of Shandong Province, Weifang Key Laboratory of Antibody Medicines, School of Bioscience and Technology, Weifang Medical University, Jinan, 261053, Shandong, China. lbwwbl@126.com.ORCID http://orcid.org/0000-0002-1261-0450
Yanran LiuSchool of Basic Medicine, Qingdao University, Qingdao, 266021, China.
Xiaodan HaoInstitute for Translational Medicine, Qingdao University, Qingdao, 266021, China.
Jinhua DongKey Laboratory of Biological Medicines in Universities of Shandong Province, Weifang Key Laboratory of Antibody Medicines, School of Bioscience and Technology, Weifang Medical University, Jinan, 261053, Shandong, China.
Limei ChenKey Laboratory of Biological Medicines in Universities of Shandong Province, Weifang Key Laboratory of Antibody Medicines, School of Bioscience and Technology, Weifang Medical University, Jinan, 261053, Shandong, China.
Haimei LiKey Laboratory of Biological Medicines in Universities of Shandong Province, Weifang Key Laboratory of Antibody Medicines, School of Bioscience and Technology, Weifang Medical University, Jinan, 261053, Shandong, China.
Wei WuCollege of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China.
Ying LiuInstitute for Translational Medicine, Qingdao University, Qingdao, 266021, China.
Jianxun WangSchool of Basic Medicine, Qingdao University, Qingdao, 266021, China.
Yin WangInstitute for Translational Medicine, Qingdao University, Qingdao, 266021, China.
Peifeng LiInstitute for Translational Medicine, Qingdao University, Qingdao, 266021, China. peifli@qdu.edu.cn.
Qingdao University · CNWeifang Medical University · CNQingdao Agricultural University · CN

Funding

China Postdoctoral Science Foundation 2018M632613Major Research Program of the National Natural Science Foundation of China 91849209National Natural Science Foundation of China 31430041
6 · The paper itself

Abstract

backgroundThe detection and identification of single nucleotide polymorphism (SNP) is essential for determining patient disease susceptibility and the delivery of medicines targeted to the individual. At present, SNP genotyping technology includes Sanger sequencing, TaqMan-probe quantitative polymerase chain reaction (qPCR), amplification-refractory mutation system (ARMS)-PCR, and Kompetitive Allele-Specific PCR (KASP). However, these technologies have some disadvantages: the high cost of development and detection, long and time consuming protocols, and high false positive rates. Focusing on these limitations, we proposed a new SNP detection method named universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR). In this method, only two types of fluorescence-labeled probes were used for SNP genotyping, thus greatly reducing the cost of development and detection for SNP genotyping.

resultsIn the amplification process of UPIP-qPCR, unlabeled intermediate primers with template-specific recognition functions could trigger probe hydrolysis and specific signal release. UPIP-qPCR can be used successfully and widely for SNP genotyping. The sensitivity of UPIP-qPCR in SNP genotyping was 0.01 ng, the call rate was more than 99.1%, and the accuracy was more than 99.9%. High-throughput DNA microarrays based on intermediate primers can be used for SNP genotyping.

conclusionThis novel approach is both cost effective and highly accurate; it is a reliable SNP genotyping method that would serve the needs of the clinician in the provision of targeted medicine.

Indexed as

Genotyping TechniquesPolymorphism, Single NucleotideAllelesGenotypeHumansReal-Time Polymerase Chain ReactionIntermediate primerSanger sequencingSNP genotypingUniversal probeUPIP-qPCR

Identifiers

PMID34819030
PMCPMC8611915
OpenAlexW3217112812

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.