ArticleApplied and environmental microbiology2022
Extracellular DNA in Environmental Samples: Occurrence, Extraction, Quantification, and Impact on Microbial Biodiversity Assessment.
Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- eDNA Metabarcoding Reveals Diel Connectivity Dynamics of Fish Communities in Xincun Lagoon, Hainan.Animals : an open access journal from MDPI · 2026Article
- A tailored MoSNational science review · 2026Article
- Pseudoalteromonas is a symbiont of marine invertebrates that exhibits broad patterns of phylosymbiosis.The ISME journal · 2026Article
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- Synthesized double stranded gene fragments are not suitable for qPCR endogenous control spike-ins.BMC research notes · 2025Article
- Progress, challenges and future directions in marine organic-walled dinoflagellate cyst research: New insights from an international workshop.Marine micropaleontology · 2025Article
- Strain-level dissection of complex rhizoplane and soil bacterial communities using single-cell genomics and metagenomics.DNA research : an international journal for rapid publication of reports on genes and genomes · 2025Article
- Soil-based environmental DNA enables detection of Oncomelania hupensis quadrasi and Schistosoma japonicum microhabitats for schistosomiasis japonica surveillance and control in the Philippines.Infectious diseases of poverty · 2025Observational
- QuickConc: A Rapid, Efficient, and Power-Free eDNA Concentration Method With Cationic-Assisted Capture.Ecology and evolution · 2025Article
- Seasonally and niche-differentiated diversity of active, dormant and dead microbes in coastal waters and surface sediments.Communications biology · 2025Article
- Article
- Passive eDNA Sampling Characterizes Fish Community Assembly in the Lancang River of Yunnan, China.Biology · 2025Article
- Changes in Microbial Community Assemblages Due To Urban Pollution, Detected via rRNA Gene Amplicon Sequencing in the Magdalena River, Mexico City.Microbial ecology · 2025Article
- Antibiotics and Antibiotic Resistance Genes in the Environment: Dissemination, Ecological Risks, and Remediation Approaches.Microorganisms · 2025Review
- Strategies Used for the Discovery of New Microbial Metabolites with Antibiotic Activity.Molecules (Basel, Switzerland) · 2025Review
- Detection of Microbial Growth on Indoor Building Materials in Two Countries Using qPCR.Microorganisms · 2025Article
- Transcriptional Difference of Deep-Sea Microorganisms under Different Sampling Methods.Environmental science & technology · 2025Article
- The performance of different methods in characterizing soil live prokaryotic diversity and abundance is highly variable.iMetaOmics · 2025Article
- Biocides as drivers of antibiotic resistance: A critical review of environmental implications and public health risks.Environmental science and ecotechnology · 2025Review
- Absolute quantification of the living skin microbiome overcomes relic-DNA bias and reveals specific patterns across volunteers.Microbiome · 2025Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental DNA, i.e., DNA extracted directly from environmental samples, has been used to understand microbial communities in the environment and to monitor contemporary biodiversity in the conservation context. Environmental DNA often contains both intracellular DNA (iDNA) and extracellular DNA (eDNA). eDNA can persist in the environment and complicate environmental DNA sequencing-based analyses of microbial communities and biodiversity. Although several studies acknowledged the impact of eDNA on DNA-based profiling of environmental communities, eDNA is still being neglected or ignored in most studies dealing with environmental samples. In this article, we summarize key findings on eDNA in environmental samples and discuss the methods used to extract and quantify eDNA as well as the importance of eDNA on the interpretation of experimental results. We then suggested several factors to consider when designing experiments and analyzing data to negate or determine the contribution of eDNA to environmental DNA-based community analyses. This field of research will be driven forward by (i) carefully designing environmental DNA extraction pipelines by taking into consideration technical details in methods for eDNA extraction/removal and membrane-based filtration and concentration; (ii) quantifying eDNA in extracted environmental DNA using multiple methods, including qPCR and fluorescent DNA binding dyes; (iii) carefully interpreting the effect of eDNA on DNA-based community analyses at different taxonomic levels; and (iv) when possible, removing eDNA from environmental samples for DNA-based community analyses.
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