ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2022
Genome-wide analysis of the Glutathione S-Transferase family in wild Medicago ruthenica and drought-tolerant breeding application of MruGSTU39 gene in cultivated alfalfa.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- From "Omics" to Field: Deciphering the Stress Adaptation Networks and Breeding Potential ofCurrent issues in molecular biology · 2026Review
- Molecular basis of drought tolerance in Rosa xanthina: an integrated analysis of physiology, transcriptome, and NAC transcription factor.BMC genomics · 2026Article
- Allelopathic Effects of Dominant Native Invaders on Forage Establishment: Implications for Alpine Meadow Restoration on the Qinghai-Xizang Plateau.Plants (Basel, Switzerland) · 2025Article
- Negatively but Not Positively Charged Nanoceria Promoted Lateral Root Growth via Modulating the Distribution of Reactive Oxygen Species Rather than Auxin.Global challenges (Hoboken, NJ) · 2025Article
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Genome-Wide Identification and Drought-Responsive Functional Analysis of theAntioxidants (Basel, Switzerland) · 2025Article
- Genome-wide identification, expression and functional analysis of glutathioneFrontiers in plant science · 2025Article
- Genome-wide identification and expression analysis of the glutathione transferase gene family and its response to abiotic stress in rye (Secale cereale).BMC genomics · 2024Article
- Genome-Wide Identification of Freezing-Responsive Genes in a Rapeseed Line NTS57 Tolerant to Low-Temperature.International journal of molecular sciences · 2024Article
- Evolution of Duplicated Glutathione Metabolic Pathway inEcology and evolution · 2024Article
- Tandem mass tag (TMT)-based quantitative proteomics analysis reveals the different responses of contrasting alfalfa varieties to drought stress.BMC genomics · 2024Article
- Transcriptome Analysis for Salt-Responsive Genes in Two Different Alfalfa (Plants (Basel, Switzerland) · 2024Article
- Identification of crucial drought-tolerant genes of barley through comparative transcriptomic analysis and yeast-based stress assay.Frontiers in genetics · 2024Article
- Article
- Priorities for the development of alfalfa pasture in northern China.Fundamental research · 2023Review
- Brassinolide promotes interaction between chloroplasts and mitochondria during the optimization of photosynthesis by the mitochondrial electron transport chain in mesophyll cell protoplasts ofFrontiers in plant science · 2023Article
- Proteomic Investigation of Molecular Mechanisms in Response to PEG-Induced Drought Stress in Soybean Roots.Plants (Basel, Switzerland) · 2022Article
- Plant hormones and neurotransmitter interactions mediate antioxidant defenses under induced oxidative stress in plants.Frontiers in plant science · 2022Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
key messageTransformation of MruGSTU39 in M. ruthenica and alfalfa enhanced growth and survival of transgenic plants by up-regulating GST and glutathione peroxidase activity to detoxify ROS under drought stress. Glutathione S-transferases (GSTs) are ubiquitous supergene family which play crucial roles in detoxification of reactive oxygen species (ROS). Despite studies on GSTs, few studies have focused on them in perennial, wild plant species with high tolerance to environmental stress. Here, we identified 66 MruGST genes from the genome of Medicago ruthenica, a perennial legume species native to temperate grasslands with high tolerance to environmental stress. These genes were divided into eight classes based on their conserved domains, phylogenetic tree and gene structure, with the tau class being the most numerous. Duplication analysis revealed that GST family in M. ruthenica was expanded by segmental and tandem duplication. Several drought-responsive MruGSTs were identified by transcriptomic analyses. Of them, expression of MruGSTU39 was up-regulated much more in a tolerant accession by drought stress. Transformation of MruGSTU39 in M. ruthenica and alfalfa (Medicago sativa) enhanced growth and survival of transgenic seedlings than their wild-type counterparts under drought. We demonstrated that MruGSTU39 can detoxify ROS to reduce its damage to membrane by up-regulating activities of GST and glutathione peroxidase. Our findings provide full-scale knowledge on GST family in the wild legume M. ruthenica with high tolerance to drought, and highlight improvement tolerance of legume forages to drought using genomic information of M. ruthenica.
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