Evidence map›Paper›PMID 34816401›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Recombinant Escherichia coli Cell Lysates as a Low-Cost Alternative for Vaccines Against Veterinary Clostridial Diseases.

Clóvis Moreira, Gustavo Marçal Schmidt Garcia Moreira, Rafael Amaral Donassolo, Marcos Roberto Alves Ferreira, Mariliana Luiza Ferreira Alves, Rafael Rodrigues Rodrigues, Fabricio Rochedo Conceição

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Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Clóvis MoreiraCentro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.
Gustavo Marçal Schmidt Garcia MoreiraInstitut fϋr Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Spielmannstr, Technische Universität Braunschweig, Braunschweig, Germany.
Rafael Amaral DonassoloCentro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.
Marcos Roberto Alves FerreiraCentro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.
Mariliana Luiza Ferreira AlvesCentro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.
Rafael Rodrigues RodriguesCentro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.
Fabricio Rochedo ConceiçãoCentro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil. fabricio.rochedo@ufpel.edu.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter describes a practical, industry-friendly, and efficient vaccine protocol based on the use of Escherichia coli cell fractions (inclusion bodies or cell lysate supernatant) containing the recombinant antigen. This approach was characterized and evaluated in laboratory and farm animals by the seroneutralization assay in mice, thereby showing to be an excellent alternative to induce a protective immune response against clostridial diseases.

Indexed as

Escherichia coli InfectionsEscherichia coli VaccinesAnimalsBacterial VaccinesEscherichia coliInclusion BodiesMiceVaccines, SyntheticBacterial VaccinesEscherichia coli VaccinesVaccines, SyntheticCell lysate supernatantClostridiosisInclusion bodiesRecombinant antigensRecombinant vaccines

Identifiers

PMID34816401

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.