ArticleNature communications2021
Mapping enzyme catalysis with metabolic biosensing.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Promiscuity-Guided Enzyme Evolution via Substrate Multiplexed Screening.Angewandte Chemie (International ed. in English) · 2026Review
- A high-throughput biocatalytic platform for screening isomeric kainoid natural products.Cell reports. Physical science · 2026Article
- Rapid analysis of terpenes produced by fermentation using flow injection analysis coupled to APCI MS.Analytical methods : advancing methods and applications · 2026Article
- Droplet microfluidics strategies for overcoming bottlenecks in antibiotics discovery.iScience · 2026Review
- Two-Layer Droplet Arrays Enable Dynamic Manipulation of Cell Microenvironment During High-Throughput Bacterial Cultivation.Small methods · 2025Article
- Bacterial Proteomics and Antibiotic Resistance Identification: Is Single-Cell Analysis a Worthwhile Pursuit?Pathogens (Basel, Switzerland) · 2025Review
- A High-Throughput Biocatalytic Platform for Screening Isomeric Kainoid Natural Products.bioRxiv : the preprint server for biology · 2025Article
- Hydrophilic/Omniphobic Droplet Arrays for High-throughput and Quantitative Enzymology.Analytical chemistry · 2025Article
- Combining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External Disturbances.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A versatile microbial platform as a tunable whole-cell chemical sensor.Nature communications · 2024Article
- High-throughput relative quantification of fatty acids by 12-plex isobaric labeling and microchip capillary electrophoresis - Mass spectrometry.Analytica chimica acta · 2024Article
- Open microfluidics: droplet microarrays as next generation multiwell plates for high throughput screening.Lab on a chip · 2024Review
- Structural Characteristics of PON1 with Leu55Met and Gln192Arg Variants Influencing Oxidative-Stress-Related Diseases: An Integrated Molecular Modeling and Dynamics Study.Medicina (Kaunas, Lithuania) · 2023Article
- Mass-Activated Droplet Sorting for the Selection of Lysine-ProducingAnalytical chemistry · 2023Article
- A combinatorial droplet microfluidic device integrated with mass spectrometry for enzyme screening.Lab on a chip · 2023Article
- Homogeneous, heterogeneous, and enzyme catalysis in microfluidics droplets.Smart molecules : open access · 2023Review
- Evaluation of Analyte Transfer between Microfluidic Droplets by Mass Spectrometry.Analytical chemistry · 2023Article
- Microbowls with Controlled Concavity for Accurate Microscale Mass Spectrometry.Advanced materials (Deerfield Beach, Fla.) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
Enzymes are represented across a vast space of protein sequences and structural forms and have activities that far exceed the best chemical catalysts; however, engineering them to have novel or enhanced activity is limited by technologies for sensing product formation. Here, we describe a general and scalable approach for characterizing enzyme activity that uses the metabolism of the host cell as a biosensor by which to infer product formation. Since different products consume different molecules in their synthesis, they perturb host metabolism in unique ways that can be measured by mass spectrometry. This provides a general way by which to sense product formation, to discover unexpected products and map the effects of mutagenesis.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.