Evidence map›Paper›PMID 34815308›Full record

ArticleGenome research2022

Comprehensive determination of transcription start sites derived from all RNA polymerases using ReCappable-seq.

Bo Yan, George Tzertzinis, Ira Schildkraut, Laurence Ettwiller

Open access · bronzeAbstract read
In one paragraph

Article in Genome research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 35 citations in OpenAlex.

  1. Review
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  9. The design and engineering of synthetic genomes.Nature reviews. Genetics · 2025
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  10. Decoding meLife · 2025
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  14. Decoding mbioRxiv : the preprint server for biology · 2025
    Article
  15. Article
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  17. Synthetic genomes unveil the effects of synonymous recoding.bioRxiv : the preprint server for biology · 2024
    Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Bo Yan *New England Biolabs Incorporated, Ipswich, Massachusetts 01938, USA.ORCID 0000-0003-0188-1113
George Tzertzinis *New England Biolabs Incorporated, Ipswich, Massachusetts 01938, USA.ORCID 0000-0001-8821-8060
Ira Schildkraut *New England Biolabs Incorporated, Ipswich, Massachusetts 01938, USA.ORCID 0000-0002-1143-6630
Laurence Ettwiller *New England Biolabs Incorporated, Ipswich, Massachusetts 01938, USA.ORCID 0000-0002-3957-6539
New England Biolabs (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Determination of eukaryotic transcription start sites (TSSs) has been based on methods that require the cap structure at the 5' end of transcripts derived from Pol II RNA polymerase. Consequently, these methods do not reveal TSSs derived from the other RNA polymerases that also play critical roles in various cell functions. To address this limitation, we developed ReCappable-seq, which comprehensively identifies TSS for both Pol II and non-Pol II transcripts at single-nucleotide resolution. The method relies on specific enzymatic exchange of 5' m

Indexed as

DNA-Directed RNA PolymerasesRNA Polymerase IIRNA, UntranslatedTranscription Initiation SiteTranscriptomeDNA-Directed RNA PolymerasesRNA Polymerase IIRNA, Untranslated

Identifiers

PMID34815308
PMCPMC8744680
OpenAlexW3216640235

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.