ArticleBiophysical journal2021
Single-molecule manipulation of macromolecules on GUV or SUV membranes using optical tweezers.
Article in Biophysical journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Nonlinear stiffness of NompC gating spring and its implication in mechanotransduction.Science advances · 2026Article
- Advancing membrane biology: single-molecule approaches meet model membrane systems.BMB reports · 2025Review
- Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Lipid osmosis, membrane tension, and other mechanochemical driving forces of lipid flow.Current opinion in cell biology · 2024Review
- The Plasma Membrane and Mechanoregulation in Cells.ACS omega · 2024Review
- Lipid osmosis, membrane tension, and other mechanochemical driving forces of lipid flow.bioRxiv : the preprint server for biology · 2024Article
- Forces of Change: Optical Tweezers in Membrane Remodeling Studies.The Journal of membrane biology · 2022Review
- A fit-less approach to the elasticity of the handles in optical tweezers experiments.European biophysics journal : EBJ · 2022Article
- Quantitative Models of Lipid Transfer and Membrane Contact Formation.Contact (Thousand Oaks (Ventura County, Calif.)) · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Despite their wide applications in soluble macromolecules, optical tweezers have rarely been used to characterize the dynamics of membrane proteins, mainly due to the lack of model membranes compatible with optical trapping. Here, we examined optical trapping and mechanical properties of two potential model membranes, giant and small unilamellar vesicles (GUVs and SUVs, respectively) for studies of membrane protein dynamics. We found that optical tweezers can stably trap GUVs containing iodixanol with controlled membrane tension. The trapped GUVs with high membrane tension can serve as a force sensor to accurately detect reversible folding of a DNA hairpin or membrane binding of synaptotagmin-1 C2AB domain attached to the GUV. We also observed that SUVs are rigid enough to resist large pulling forces and are suitable for detecting protein conformational changes induced by force. Our methodologies may facilitate single-molecule manipulation studies of membrane proteins using optical tweezers.
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Registered trials
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