ArticleACS applied materials & interfaces2021
Streptavidin Coverage on Biotinylated Surfaces.
Article in ACS applied materials & interfaces, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
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- Generation of aptamers for the selective detection and neutralization of soluble lymphotoxin alpha.Molecular therapy. Nucleic acids · 2026Article
- Designing the Future of Biosensing: Advances in Aptamer Discovery, Computational Modeling, and Diagnostic Applications.Biosensors · 2025Review
- Quantitative Detection of Biological Nanoparticles Using Twilight Off-Axis Holographic Microscopy: Insights on Complex Formation between PEGylated Gold Nanoparticles and Lipid Vesicles.The journal of physical chemistry. B · 2025Article
- Quantifying and Controlling DNA Probe Density on the Surface of Silicon Nitride Optical Waveguides.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Analyzing Binding Specificity in a Microparticle-Based DNA Displacement Assay Using Multiharmonic QCM-D.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Molecular fingerprinting of biological nanoparticles with a label-free optofluidic platform.Nature communications · 2024Article
- Competitive Specific Anchorage of Molecules onto Surfaces: Quantitative Control of Grafting Densities and Contamination by Free Anchors.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Molecular fingerprinting of biological nanoparticles with a label-free optofluidic platform.Research square · 2023Article
- Protein nanoarrays using the annexin A5 two-dimensional crystal on supported lipid bilayers.Nanoscale advances · 2023Article
- QCM-D Investigations on Cholesterol-DNA Tethering of Liposomes to Microbubbles for Therapy.The journal of physical chemistry. B · 2023Article
- Kinetic Analysis of Prostate-Specific Antigen Interaction with Monoclonal Antibodies for Development of a Magnetic Immunoassay Based on Nontransparent Fiber Structures.Molecules (Basel, Switzerland) · 2022Article
- Density Control over MBD2 Receptor-Coated Surfaces Provides Superselective Binding of Hypermethylated DNA.ACS applied materials & interfaces · 2022Article
- Distinct Binding Properties of Neutravidin and Streptavidin Proteins to Biotinylated Supported Lipid Bilayers: Implications for Sensor Functionalization.Sensors (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biosensors and other biological platform technologies require the functionalization of their surface with receptors to enhance affinity and selectivity. Control over the functionalization density is required to tune the platform's properties. Streptavidin (SAv) monolayers are widely used to immobilize biotinylated proteins, receptors, and DNA. The SAv density on a surface can be varied easily, but the predictability is dependent on the method by which the SAv is immobilized. In this study we show a method to quantitatively predict the SAv coverage on biotinylated surfaces. The method is validated by measuring the SAv coverage on supported lipid bilayers with a range of biotin contents and two different main phase lipids and by using quartz crystal microbalance and localized surface plasmon resonance. We explore a predictive model of the biotin-dependent SAv coverage without any fit parameters. Model and data allow to predict the SAv coverage based on the biotin coverage, in both the low- and high-density regimes. This is of special importance in applications with multivalent binding where control over surface receptor density is required, but a direct measurement is not possible.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.