Evidence map›Paper›PMID 34811553›Full record

ArticleNature nanotechnology2022

Nanoparticle cellular internalization is not required for RNA delivery to mature plant leaves.

Huan Zhang, Natalie S Goh, Jeffrey W Wang, Rebecca L Pinals, Eduardo González-Grandío, Gozde S Demirer, Salwan Butrus, Sirine C Fakra, Antonio Del Rio Flores, Rui Zhai and 3 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature nanotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.

0numbers the graph read from it
0cells of the map it votes in
80citing papers in PubMed
10.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

80 citing papers in PubMed, 208 citations in OpenAlex.

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20 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 7 institutions in 3 countries.

Huan Zhang *Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
Natalie S Goh *Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
Jeffrey W WangDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
Rebecca L PinalsDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-5369-2317
Eduardo González-GrandíoDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-2059-4542
Gozde S DemirerDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0002-3007-1489
Salwan ButrusDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.
Sirine C FakraAdvanced Light Source, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Antonio Del Rio FloresDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0001-5893-3557
Rui ZhaiDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA.ORCID http://orcid.org/0000-0001-5032-9689
Bin ZhaoSchool of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou, China.
So-Jung ParkDepartment of Chemistry and Nanoscience, Ewha Womans University, Seoul, Republic of Korea.
Markita P LandryDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA, USA. landry@berkeley.edu.ORCID http://orcid.org/0000-0002-5832-8522
University of California, Berkeley · USEwha Womans University · KRJinan University · CNLawrence Berkeley National Laboratory · USQB3 · USSouth China University of Technology · CNUniversity of California, Davis · US

Funding

Single-Molecule Imaging of Biological Trauma:Cytokine-Based Intracellular CommunicationR35GM128922 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI LANDRY, MARKITA · 2019 to 2023
$2.0M
NIGMS NIH HHS R35 GM128922
6 · The paper itself

Abstract

Rapidly growing interest in the nanoparticle-mediated delivery of DNA and RNA to plants requires a better understanding of how nanoparticles and their cargoes translocate in plant tissues and into plant cells. However, little is known about how the size and shape of nanoparticles influence transport in plants and the delivery efficiency of their cargoes, limiting the development of nanotechnology in plant systems. In this study we employed non-biolistically delivered DNA-modified gold nanoparticles (AuNPs) of various sizes (5-20 nm) and shapes (spheres and rods) to systematically investigate their transport following infiltration into Nicotiana benthamiana leaves. Generally, smaller AuNPs demonstrated more rapid, higher and longer-lasting levels of association with plant cell walls compared with larger AuNPs. We observed internalization of rod-shaped but not spherical AuNPs into plant cells, yet, surprisingly, 10 nm spherical AuNPs functionalized with small-interfering RNA (siRNA) were the most efficient at siRNA delivery and inducing gene silencing in mature plant leaves. These results indicate the importance of nanoparticle size in efficient biomolecule delivery and, counterintuitively, demonstrate that efficient cargo delivery is possible and potentially optimal in the absence of nanoparticle cellular internalization. Overall, our results highlight nanoparticle features of importance for transport within plant tissues, providing a mechanistic overview of how nanoparticles can be designed to achieve efficacious biocargo delivery for future developments in plant nanobiotechnology.

Indexed as

DNAGene SilencingGene Transfer TechniquesGoldMetal NanoparticlesNicotianaPlant LeavesRNA, Small InterferingDNAGoldRNA, Small Interfering

Identifiers

PMID34811553
PMCPMC10519342
OpenAlexW3217192743

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.