ArticleScientific reports2021
Quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Birth of offspring derived from cryopreserved rat sperm after shipment in a Styrofoam box at -80°C.Experimental animals · 2025Article
- Improved protocol for the vitrification and warming of rat zygotes by optimizing the warming solution and oocyte donor age.PloS one · 2025Article
- In vivo fertilization improved the cryotolerance and developmental ability of vitrified-warmed rat fertilized oocytes.Scientific reports · 2024Article
- High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm.The Journal of reproduction and development · 2024Article
- In vitro fertilization using sperm activated by ML-2-3 isolated fromReproductive medicine and biologyArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Shipment of laboratory rats between animal facilities is frequently performed using special containers. However, the shipment of live animals is associated with potential risks of infectious diseases, escape and death during shipment and animal welfare issues. The transport of cold-stored sperm avoids such risks; however, there have been no reports on cold storage of rat sperm. We previously reported that dimethyl sulfoxide (DMSO) and quercetin maintained the motility and fertilising abilities of cold-stored mouse sperm stored for 10 days. The present study investigated the efficacy of DMSO and quercetin in the cold storage of rat sperm. Quercetin maintained motility and fertility of cold-stored rat sperm stored for 5 days. After in vitro fertilisation using cold-stored sperm, pronuclear and two-cell embryos developed normally to pups following embryo transfer. Therefore, we demonstrated that live pups could be obtained from sperm transported using the cold-storage system. We conclude that cold storage of rat sperm may provide an efficient system for transporting rat resources as an alternative to shipping live animals.
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