ArticleMolecular and cellular biology1987
Expression of transfected vimentin genes in differentiating murine erythroleukemia cells reveals divergent cis-acting regulation of avian and mammalian vimentin sequences.
Article in Molecular and cellular biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Vimentin expression is retained in erythroid cells differentiated from human iPSC and ESC and indicates dysregulation in these cells early in differentiation.Stem cell research & therapy · 2019Article
- Adult chicken alpha-globin gene expression in transfected QT6 quail cells: evidence for a negative regulatory element in the alpha D gene region.Nucleic acids research · 1991Article
- Desmin/vimentin intermediate filaments are dispensable for many aspects of myogenesis.The Journal of cell biology · 1991Article
- Regulation of the expression of vimentin gene during the differentiation of mouse myeloid leukemia cells.The Journal of cell biology · 1990Article
- Expression of the chicken vimentin gene in transgenic mice: efficient assembly of the avian protein into the cytoskeleton.Proceedings of the National Academy of Sciences of the United States of America · 1989Article
- Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells.The Journal of cell biology · 1989Article
- Enhancer elements directing cell-type-specific expression of cytokeratin genes and changes of the epithelial cytoskeleton by transfections of hybrid cytokeratin genes.The EMBO journal · 1989Article
- Concentration dependence of transcriptional transactivation in inducible E1A-containing human cells.Molecular and cellular biology · 1988Article
- Tissue-specific expression of a vimentin--desmin hybrid gene in transgenic mice.The EMBO journal · 1988Article
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4 authors at 1 institution in 1 country.
Funding
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Abstract
We studied the expression of transfected chicken and hamster vimentin genes in murine erythroleukemia (MEL) cells. MEL cells normally repress the levels of endogenous mouse vimentin mRNA during inducermediated differentiation, resulting in a subsequent loss of vimentin filaments. Expression of vimentin in differentiating MEL cells reflects the disappearance of vimentin filaments during mammalian erythropoiesis in vivo. In contrast, chicken erythroid cells express high levels of vimentin mRNA and vimentin filaments during terminal differentiation. We demonstrate here that chicken vimentin mRNA levels increase significantly in differentiating transfected MEL cells, whereas similarly transfected hamster vimentin genes are negatively regulated. In conjunction with in vitro nuclear run-on transcription experiments, these results suggest that the difference in vimentin expression in avian and mammalian erythropoiesis is due to a divergence of cis-linked vimentin sequences that are responsible for transcriptional and posttranscriptional regulation of vimentin gene expression. Transfected chicken vimentin genes produce functional vimentin protein and stable vimentin filaments during MEL cell differentiation, further demonstrating that the accumulation of vimentin filaments is determined by the abundance of newly synthesized vimentin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.