ArticleGenome biology2021
Transcriptional landscape of highly lignified poplar stems at single-cell resolution.
Article in Genome biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed, 112 citations in OpenAlex.
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- Integrating scRNA-seq and snRNA-seq with spatial transcriptomics to unlock the xylem puzzle.Genome biology · 2026Article
- Identification of key regulators for the development of Prunus leaves using single-cell RNA sequencing.BMC plant biology · 2026Article
- Integrated single-cell transcriptomics and spatial metabolomics unveil cellular differentiation and ginsenosides biosynthesis inHorticulture research · 2025Article
- The GoSPGF-GoPGF-GhWRKYs transcriptional cascade positively regulates pigment gland formation in the stem of upland cotton.Plant biotechnology journal · 2025Article
- Single-nucleus transcriptomics revealed auxin-driven mechanisms of wood plasticity to enhance severe drought tolerance in poplar.Genome biology · 2025Article
- Deep tissue profiling of Populus stem at single nucleus level reveals uncharacterized cell types and cell-specific gene regulatory networks.Genome biology · 2025Article
- Single-cell transcriptome sequencing reveals the sphingolipid metabolism pathway plays an important role in leaf senescence in tobacco.BMC plant biology · 2025Article
- Validation of Fiber-Dominant Expressing Gene Promoters inPlants (Basel, Switzerland) · 2025Article
- Developmental landscape and asymmetric gene expression in the leaf vasculature ofHorticulture research · 2025Article
- Four near-complete genome assemblies reveal the landscape and evolution of centromeres in Salicaceae.Genome biology · 2025Article
- Identification of phloem-specific proteinaSEOus structure heterogeneity in sieve element of Populus trichocarpa.BMC plant biology · 2025Article
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlant secondary growth depends on the activity of the vascular cambium, which produces xylem and phloem. Wood derived from xylem is the most abundant form of biomass globally and has played key socio-economic and subsistence roles throughout human history. However, despite intensive study of vascular development, the full diversity of cell types and the gene networks engaged are still poorly understood.
resultsHere, we have applied an optimized protoplast isolation protocol and RNA sequencing to characterize the high-resolution single-cell transcriptional landscape of highly lignified poplar stems. We identify 20 putative cell clusters with a series of novel cluster-specific marker genes and find that these cells are highly heterogeneous based on the transcriptome. Analysis of these marker genes' expression dynamics enables reconstruction of the cell differentiation trajectories involved in phloem and xylem development. We find that different cell clusters exhibit distinct patterns of phytohormone responses and emphasize the use of our data to predict potential gene redundancy and identify candidate genes related to vascular development in trees.
conclusionsThese findings establish the transcriptional landscape of major cell types of poplar stems at single-cell resolution and provide a valuable resource for investigating basic principles of vascular cell specification and differentiation in trees.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.