ArticleThe plant genome2022
Accumulation of somatic mutations leads to genetic mosaicism in cannabis.
Article in The plant genome, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- Cannabis: from crop to shop-some insights about stability to access quality control.Journal of cannabis research · 2026Review
- Micropropagation of Cannabis sativa: genetic and epigenetic stability assessment over multiple generations.Journal of cannabis research · 2026Article
- Importance of Media Composition and Explant Type inPlants (Basel, Switzerland) · 2024Article
- Somatic Mutation Accumulations in Micropropagated Cannabis Are Proportional to the Number of Subcultures.Plants (Basel, Switzerland) · 2024Article
- Characterization of indigenous populations of cannabis in Iran: a morphological and phenological study.BMC plant biology · 2024Article
- Comparative restriction enzyme analysis of methylation (CREAM) reveals methylome variability within a clonalFrontiers in plant science · 2024Article
- Challenges toInternational journal of molecular sciences · 2023Article
- Comprehensive Phenotypic Characterization of Diverse Drug-TypePlants (Basel, Switzerland) · 2023Article
- Effect of Explant Source on Phenotypic Changes of In Vitro Grown Cannabis Plantlets over Multiple Subcultures.Biology · 2023Article
- Challenges and potentials of new breeding techniques inFrontiers in plant science · 2023Review
- Somatic mutations during rapid clonal domestication ofEvolutionary applications · 2022Article
- New Insight into Ornamental Applications of Cannabis: Perspectives and Challenges.Plants (Basel, Switzerland) · 2022Article
- In vitro plant tissue culture as the fifth generation of bioenergy.Scientific reports · 2022Article
- Accumulation of somatic mutations leads to genetic mosaicism in cannabis.The plant genome · 2022Article
- Impact of Harvest Time and Pruning Technique on Total CBD Concentration and Yield of Medicinal Cannabis.Plants (Basel, Switzerland) · 2022Article
- Cryopreservation of 13 CommercialPlants (Basel, Switzerland) · 2021Article
- Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis.International journal of molecular sciences · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cannabis (Cannabis sativa L.) is typically propagated using stem cuttings taken from mother plants to produce genetically uniform propagules. However, producers anecdotally report that clonal lines deteriorate over time and eventually produce clones with less vigor and lower cannabinoid levels than the original mother plant. While the cause of this deterioration has not been investigated, one potential contributor is the accumulation of somatic mutations within the plant. To test this, we used deep sequencing of whole genomes (>50×) to compare the variability within an individual cannabis cultivar Honey Banana plant sampled at the bottom, middle, and top. We called over six million sequence variants based on a reference genome and found that the top had the most by a sizable amount. Comparing the variants among the samples uncovered that nearly 600,000 (34%) were unique to the top while the bottom only contained 148,000 (12%), and middle with 77,000 (9%) unique variants. Bioinformatics tools were used to identify mutations in critical cannabinoid-terpene biosynthesis pathways. While none were identified as high impact, four genes contained more than double the average level of nucleotide diversity (π) in or near the gene. Two genes code for essential enzymes required for the cannabinoid pathway while the other two are in the terpene pathways, demonstrating that mutations were accumulating within these pathways and could influence their function. Overall, a measurable number of intraplant genetic diversity was discovered that could impact long-term genetic fidelity of clonal lines and potentially contribute to the observed decline in vigor and cannabinoid content.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.