ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2022
Directed Regeneration of Osteochondral Tissue by Hierarchical Assembly of Spatially Organized Composite Spheroids.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 60 citations in OpenAlex.
- Synchronizing the Osteochondral Regeneration Process through Spatial Patterning of Stable and Hypertrophic Cartilage Organoids.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- A Microfiber-Reinforced Janus Hydrogel E-Skin With Recyclable Feature for Multimodal Sensing and Gender-Specific Physiological Monitoring.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Advancing the 3Rs in bone tissue engineering: emergingFrontiers in physiology · 2026Review
- Engineered 3D mesenchymal stem cell aggregates with multifunctional prowess for bone regeneration: Current status and future prospects.Journal of advanced research · 2026Review
- Programmable Macrophage Mimics for Inflammatory Meniscus Regeneration via Nanotherapy.Research (Washington, D.C.) · 2026Article
- Integrated 3D printing of topologically hierarchical mechanical hydrogel for accelerating osteochondral regeneration.Bioactive materials · 2026Article
- Anti-swelling and self-activating artificial proteoglycan assemblies as cartilaginous implant for post-microfracture healing.Nature communications · 2025Article
- Cryogenic 3D printing of heterogeneous scaffolds with capability to spatially tune cellular morphology of mesenchymal stem cells for integrated osteochondral regeneration.Materials today. Bio · 2025Article
- Intelligent microstructure materials for diagnosis and treatment of osteoarthritis: progress and AI-enpowered future.Bone research · 2025Review
- Establishment and characterization of an inflammatory cartilaginous organoids model for organoid transplantation study.Journal of orthopaedic translation · 2025Article
- Research advance of 3D printing for articular cartilage regeneration.Regenerative medicine · 2025Review
- Advances in Additive Manufactured Scaffolds Mimicking the Osteochondral Interface.Advanced nanobiomed research · 2024Article
- 3D Niche-Inspired Scaffolds as a Stem Cell Delivery System for the Regeneration of the Osteochondral Interface.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Melatonin Delays Arthritis Inflammation and Reduces Cartilage Matrix Degradation through the SIRT1-Mediated NF-κB/Nrf2/TGF-β/BMPs Pathway.International journal of molecular sciences · 2024Article
- Enhanced osteochondral regeneration with a 3D-Printed biomimetic scaffold featuring a calcified interfacial layer.Bioactive materials · 2024Article
- Article
- 3D printed osteochondral scaffolds: design strategies, present applications and future perspectives.Frontiers in bioengineering and biotechnology · 2024Review
- Advancements in culture technology of adipose-derived stromal/stem cells: implications for diabetes and its complications.Frontiers in endocrinology · 2024Review
- Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel.Journal of biological engineering · 2023Article
- A Synergic Strategy: Adipose-Derived Stem Cell Spheroids Seeded on 3D-Printed PLA/CHA Scaffolds Implanted in a Bone Critical-Size Defect Model.Journal of functional biomaterials · 2023Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The use of engineered scaffolds or stem cells is investigated widely in the repair of injured musculoskeletal tissue. However, the combined regeneration of hierarchical osteochondral tissue remains a challenge due to delamination between cartilage and subchondral bone or difficulty in spatial control over differentiation of transplanted stem cells. Here, two types of composite spheroids are prepared using adipose-derived stem cells (hADSCs) and nanofibers coated with either transforming growth factor-β3 or bone morphogenetic growth factor-2 for chondrogenesis or osteogenesis, respectively. Each type of spheroid is then cultured within a 3D-printed microchamber in a spatially arranged manner to recapitulate the bilayer structure of osteochondral tissue. The presence of inductive factors regionally modulates in vitro chondrogenic or osteogenic differentiation of hADSCs within the biphasic construct without dedifferentiation. Furthermore, hADSCs from each spheroid proliferate and sprout and successfully connect the two layers mimicking the osteochondral interface without apertures. In vivo transplantation of the biphasic construct onto a femoral trochlear groove defect in rabbit knee joint results in 21.2 ± 2.8% subchondral bone volume/total volume and a cartilage score of 25.0 ± 3.7. The present approach can be an effective therapeutic platform to engineer complex tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.