Evidence map›Paper›PMID 34806156›Full record

ArticleJournal of bioenergetics and biomembranes2022

microRNA-15b-5p shuttled by mesenchymal stem cell-derived extracellular vesicles protects podocytes from diabetic nephropathy via downregulation of VEGF/PDK4 axis.

Tiantian Zhao, Qingsong Jin, Lili Kong, Dongdong Zhang, Yaqin Teng, Liangyan Lin, Xiaoyan Yao, Yongjun Jin, Minglong Li

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of bioenergetics and biomembranes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 38 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Tiantian ZhaoDepartment of Endocrinology and Geriatrics, Shandong Provincial Hospital Affiliated to Shandong University, No. 324, Jingwu Road, Jinan, 250021, Shandong Province, China.
Qingsong JinDepartment of Endocrinology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264100, China.
Lili KongDepartment of Endocrinology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264100, China.
Dongdong ZhangDepartment of Endocrinology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264100, China.
Yaqin TengDepartment of Endocrinology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264100, China.
Liangyan LinDepartment of Endocrinology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264100, China.
Xiaoyan YaoDepartment of Endocrinology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264100, China.
Yongjun JinDepartment of Endocrinology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, 264100, China.
Minglong LiDepartment of Endocrinology and Geriatrics, Shandong Provincial Hospital Affiliated to Shandong University, No. 324, Jingwu Road, Jinan, 250021, Shandong Province, China. 15168887159@163.com.
Binzhou Medical University · CNBinzhou University · CNShandong Provincial Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Diabetic nephropathy (DN) is a severe complication of diabetes lethal for end-stage renal disease, with less treatment methodologies and uncertain pathogenesis. In the current study, we determined the role of mesenchymal stem cells (MSCs)-derived extracellular vesicles (EVs) containing microRNA (miR)-15b-5p in DN. After extraction and identification of MSC-derived EVs, mouse podocyte line MPC5 was selected to establish an in vitro high-glucose (HG) cell model, where expression of miR-15b-5p, pyruvate dehydrogenase kinase 4 (PDK4) and VEGFA expression in tissues and cells were determined. The loss- and gain- function assays were conducted to determine the roles of miR-15b-5p, PDK4 and VEGFA. MPC5 cells were then co-cultured with MSC-derived EVs and their biological behaviors were detected by Western blot, CCK-8 assay, and flow cytometry. The binding relationship between miR-15b-5p and PDK43 by dual luciferase reporter gene assay. The expression of miR-15b-5p was downregulated in podocytes under HG environment, but highly expressed in mouse MSCs-derived EVs. EVs-derived miR-15b-5p could protect MPC5 cell apoptosis and inflammation. miR-15b-5p inhibited the expression of PDK4 by directly bound to the 3'UTR region of PDK4 gene. miR-15b-5p inhibits VEGF expression by binding to PDK4. Inhibition of PDK4 decreased VEGFA expression and reduced apoptosis and inflammation. Collectively, miR-15b-5p shuttled by MSC-derived EV can play protective roles in HG-induced mouse podocyte injury, possibly by targeting PDK4 and decreasing the VEGFA expression.

Indexed as

Diabetes MellitusDiabetic NephropathiesExtracellular VesiclesMesenchymal Stem CellsMicroRNAsPodocytesAnimalsDown-RegulationGlucoseMiceProtein KinasesVascular Endothelial Growth Factor AGlucoseMicroRNAsProtein Kinasespyruvate dehydrogenase kinase 4Vascular Endothelial Growth Factor AApoptosisDiabetesEVsInflammationmiR-15b-5pMPC5 cellMSCsPDK4VEGFA

Identifiers

PMID34806156
OpenAlexW3216183409

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.