Evidence map›Paper›PMID 34806048›Full record

ArticleSTAR protocols2021

Marker-based assays for studying gut transit in gnotobiotic and conventional mouse models.

Sean T Koester, Naisi Li, Daniel M Lachance, Neelendu Dey

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 21 citations in OpenAlex.

  1. A noninvasiveAmerican journal of physiology. Endocrinology and metabolism · 2026
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  7. Acoustic percolation switches enable targeted drug delivery controlled by diagnostic ultrasound.Proceedings of the National Academy of Sciences of the United States of America · 2025
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  8. Benefits ofInternational journal of molecular sciences · 2025
    Article
  9. Article
  10. Frontiers in neuroscience · 2025
    Article
  11. Article
  12. Article
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  15. Article
  16. RET is a sex-biased regulator of intestinal tumorigenesis.Frontiers in gastroenterology (Lausanne, Switzerland) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Sean T KoesterClinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Naisi LiClinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Daniel M LachanceClinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Neelendu DeyClinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Fred Hutch Cancer Center · USUniversity of Washington · US

Funding

Translational Bioimaging Core Shared ResourceP30CA015704 · NCI · FRED HUTCHINSON CANCER RESEARCH CENTER · PI Marian Esvelt · 1985 to 2026
$296.4M
The gut microbiota, bile acid-mediated enteric nervous system signaling, and modulation of gastrointestinal motilityK08DK111941 · NIDDK · WASHINGTON UNIVERSITY · PI DEY, NEELENDU · 2016 to 2020
$809k
NCI NIH HHS P30 CA015704NIDDK NIH HHS K08 DK111941
6 · The paper itself

Abstract

Gastrointestinal motility is regulated by a variety of environmental factors including gut microbes and metabolites. The ability to interrogate mouse models of gut motility has enabled elucidation of these relationships. Here we describe integration of the red carmine dye and fluorescence isothiocyanate-dextran marker-based assays for characterizing gut transit with spatial resolution, along with an optional intracolonic infusion protocol for studying the effects of metabolites on colonic transit. These protocols can be adapted for use in gnotobiotic and conventional mouse models. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).

Indexed as

Disease Models, AnimalAnimalsBiomarkersFecesFemaleGastrointestinal TractGastrointestinal TransitGerm-Free LifeMaleMiceBiomarkersMicrobiologyModel Organisms

Identifiers

PMID34806048
PMCPMC8585659
OpenAlexW3212781357

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.