Evidence map›Paper›PMID 34806047›Full record

ArticleSTAR protocols2021

Protocol for design, construction, and selection of genome phage (gPhage) display libraries.

Luis Antonio Rodriguez Carnero, André Azevedo Reis Teixeira, Fenny Hui Fen Tang, Andréia Kuramoto, Maria Júlia Manso Alves, Walter Colli, João Carlos Setubal, Edécio Cunha-Neto, Renata Pasqualini, Wadih Arap and 1 more

Abstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Luis Antonio Rodriguez CarneroDepartment of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil.
André Azevedo Reis TeixeiraDepartment of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil.
Fenny Hui Fen TangDepartment of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil.
Andréia KuramotoHeart Institute (InCor), University of São Paulo School of Medicine, São Paulo, SP 05403, Brazil.
Maria Júlia Manso AlvesDepartment of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil.
Walter ColliDepartment of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil.
João Carlos SetubalDepartment of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil.
Edécio Cunha-NetoHeart Institute (InCor), University of São Paulo School of Medicine, São Paulo, SP 05403, Brazil.
Renata PasqualiniRutgers Cancer Institute of New Jersey, Newark, NJ 07101, USA.
Wadih ArapRutgers Cancer Institute of New Jersey, Newark, NJ 07101, USA.
Ricardo José GiordanoDepartment of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism,

Indexed as

Genomic LibraryAntibodies, ProtozoanCell Surface Display TechniquesGenome, ProtozoanTrypanosoma cruziAntibodies, ProtozoanAntibodyGenomicsHigh Throughput ScreeningImmunologyMicrobiologyModel OrganismsMolecular BiologyMolecular/Chemical ProbesSequence analysisSequencing

Identifiers

PMID34806047
PMCPMC8590080

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.