Evidence map›Paper›PMID 34804544›Full record

ArticleIUCrJ2021

Pink-beam serial femtosecond crystallography for accurate structure-factor determination at an X-ray free-electron laser.

Karol Nass, Camila Bacellar, Claudio Cirelli, Florian Dworkowski, Yaroslav Gevorkov, Daniel James, Philip J M Johnson, Demet Kekilli, Gregor Knopp, Isabelle Martiel and 8 more

Abstract read
In one paragraph

Article in IUCrJ, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Convergent-beam attosecond x-ray crystallography.Structural dynamics (Melville, N.Y.) · 2025
    Article
  5. Article
  6. Article
  7. Article
  8. Journal of applied crystallography · 2024
    Article
  9. Scaling and Merging Time-Resolved Laue Data with Variational Inference.bioRxiv : the preprint server for biology · 2024
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Karol NassPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.ORCID https://orcid.org/0000-0001-8843-1566
Camila BacellarPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.ORCID https://orcid.org/0000-0003-2166-241X
Claudio CirelliPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.ORCID https://orcid.org/0000-0003-4576-3805
Florian DworkowskiPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.ORCID https://orcid.org/0000-0001-5004-8684
Yaroslav GevorkovCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, Hamburg 22607, Germany.
Daniel JamesPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.ORCID https://orcid.org/0000-0002-8348-6661
Philip J M JohnsonPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.ORCID https://orcid.org/0000-0002-7251-4815
Demet KekilliPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.
Gregor KnoppPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.ORCID https://orcid.org/0000-0002-0786-3402
Isabelle MartielPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.ORCID https://orcid.org/0000-0003-1318-3830
Dmitry OzerovPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.ORCID https://orcid.org/0000-0002-3274-1124
Alexandra TolstikovaCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, Hamburg 22607, Germany.
Laura VeraPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.ORCID https://orcid.org/0000-0003-3804-6314
Tobias WeinertPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.
Oleksandr YefanovCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, Hamburg 22607, Germany.ORCID https://orcid.org/0000-0001-8676-0091
Jörg StandfussPaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.
Sven ReichePaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.ORCID https://orcid.org/0000-0002-7797-6736
Christopher J MilnePaul Scherrer Institut, Forschungstrasse 111, Villigen 5232, Switzerland.ORCID https://orcid.org/0000-0003-4714-9139

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables essentially radiation-damage-free macromolecular structure determination using microcrystals that are too small for synchrotron studies. However, SFX experiments often require large amounts of sample in order to collect highly redundant data where some of the many stochastic errors can be averaged out to determine accurate structure-factor amplitudes. In this work, the capability of the Swiss X-ray free-electron laser (SwissFEL) was used to generate large-bandwidth X-ray pulses [Δλ/λ = 2.2% full width at half-maximum (FWHM)], which were applied in SFX with the aim of improving the partiality of Bragg spots and thus decreasing sample consumption while maintaining the data quality. Sensitive data-quality indicators such as anomalous signal from native thaumatin micro-crystals and

Indexed as

data-quality indicatorsde novo protein structure determinationlarge bandwidthspink beamsserial femtosecond crystallographysingle-wavelength anomalous diffractionXFELs

Identifiers

PMID34804544
PMCPMC8562661

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.