ArticleIUCrJ2021
High-resolution single-particle cryo-EM of samples vitrified in boiling nitro-gen.
Article in IUCrJ, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Reaching the full potential of cryo-EM reconstructions with molecular dynamics simulations at 310 K: Actin filaments as an example.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Comprehensive microcrystal electron diffraction sample preparation for cryo-EM.Nature protocols · 2025Review
- Ice formation and its elimination in cryopreservation of oocytes.Scientific reports · 2024Article
- Determining Protein Secondary Structures in Heterogeneous Medium-Resolution Cryo-EM Images Using CryoSSESeg.ACS omega · 2024Article
- Ice formation and its elimination in cryopreservation of oocytes.Research square · 2024Article
- VitroJet: new features and case studies.Acta crystallographica. Section D, Structural biology · 2024Article
- C-SPAM: an open-source time-resolved specimen vitrification device with light-activated molecules.IUCrJ · 2024Article
- Folding of prestin's anion-binding site and the mechanism of outer hair cell electromotility.eLife · 2023Article
- Visualizing lipid membrane structure with cryo-EM: past, present, and future.Emerging topics in life sciences · 2023Review
- Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA.Nature communications · 2023Article
- Determining biomolecular structures near room temperature using X-ray crystallography: concepts, methods and future optimization.Acta crystallographica. Section D, Structural biology · 2023Article
- Monovalent ion-mediated charge-charge interactions drive aggregation of surface-functionalized gold nanoparticles.Nanoscale · 2022Article
- On the reduction in the effects of radiation damage to two-dimensional crystals of organic and biological molecules at liquid-helium temperature.Ultramicroscopy · 2022Article
- Microsecond melting and revitrification of cryo samples: protein structure and beam-induced motion.Acta crystallographica. Section D, Structural biology · 2022Article
- Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.Annual review of biochemistry · 2022Review
- Cryo-SEM and confocal LSM studies of agar gel, nanoparticle hydrocolloid, mineral clays and saline solutions.Scientific reports · 2022Article
- Effects of cryo-EM cooling on structural ensembles.Nature communications · 2022Article
- A case for glycerol as an acceptable additive for single-particle cryoEM samples.Acta crystallographica. Section D, Structural biology · 2022Article
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Based on work by Dubochet and others in the 1980s and 1990s, samples for single-particle cryo-electron microscopy (cryo-EM) have been vitrified using ethane, propane or ethane/propane mixtures. These liquid cryogens have a large difference between their melting and boiling temperatures and so can absorb substantial heat without formation of an insulating vapor layer adjacent to a cooling sample. However, ethane and propane are flammable, they must be liquified in liquid nitro-gen immediately before cryo-EM sample preparation, and cryocooled samples must be transferred to liquid nitro-gen for storage, complicating workflows and increasing the chance of sample damage during handling. Experiments over the last 15 years have shown that cooling rates required to vitrify pure water are only ∼250 000 K s
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.