ArticleBiophysical journal2022
Dedifferentiation alters chondrocyte nuclear mechanics during in vitro culture and expansion.
Article in Biophysical journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 41 citations in OpenAlex.
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- Deformation Microscopy: A Protocol for Full-Field, Micro- to Nano-Scale Imaging of Mechanics in Extracellular Matrix, Cells, and Nuclei.Current protocols · 2026Article
- Reprogramming Dedifferentiation Regulatory Networks Preserves Human Chondrocyte Phenotypes.bioRxiv : the preprint server for biology · 2026Article
- Microcavitary Alginate Hydrogel Reverses Chondrocyte Dedifferentiation via Temporal Modulation of the p53 Pathway.Annals of biomedical engineering · 2026Article
- A Comprehensive Review of Nuclear Mechanics: Advances, Disease Relevance, Methodologies, and AI Applications.Cell biochemistry and biophysics · 2026Review
- Chondrogenic potential of mesenchymal progenitors from somatic and cartilage-derived iPSCs is predicted by their transcriptomic signatures.Genes & diseases · 2026Article
- Cell phenotypes in the biomedical literature: a systematic analysis and text mining corpus.bioRxiv : the preprint server for biology · 2026Article
- Ultrasound stimulation increases cartilage synthesis and revert dedifferentiation on human chondrocytes.Scientific reports · 2026Article
- Expansion and Delivery of Human Chondrocytes on Gelatin-Based Cell Carriers.Gels (Basel, Switzerland) · 2025Article
- Active matter in the nucleus: Chromatin remodeling drives nuclear force dissipation.Biophysical journal · 2025Article
- Decellularized Cell-Secreted Extracellular Matrices as Biomaterials for Tissue Engineering.Small science · 2025Review
- Mesangiogenic progenitor cells: a mesengenic and vasculogenic branch of hemopoiesis? A story of neglected plasticity.Frontiers in cell and developmental biology · 2025Article
- The intersection of aging and estrogen in osteoarthritis.npj women's health · 2025Review
- Spatiotemporal analysis of multi-scale cell structure in spheroid culture reveals hypertrophic chondrocyte differentiation.Cell and tissue research · 2024Article
- LGR5 Modulates Differentiated Phenotypes of Chondrocytes Through PI3K/AKT Signaling Pathway.Tissue engineering and regenerative medicine · 2024Article
- A high throughput cell stretch device for investigating mechanobiologyAPL bioengineering · 2024Article
- Microscopy methods to visualize nuclear organization in biomechanical studies.Current opinion in biomedical engineering · 2024Article
- Epigenetic Priming Enhances Chondrogenic Potential of Expanded Chondrocytes.Tissue engineering. Part A · 2024Article
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9 authors at 3 institutions in 1 country.
Funding
Abstract
The biophysical features of a cell can provide global insights into diverse molecular changes, especially in processes like the dedifferentiation of chondrocytes. Key biophysical markers of chondrocyte dedifferentiation include flattened cellular morphology and increased stress-fiber formation. During cartilage regeneration procedures, dedifferentiation of chondrocytes during in vitro expansion presents a critical limitation to the successful repair of cartilage tissue. Our study investigates how biophysical changes of chondrocytes during dedifferentiation influence the nuclear mechanics and gene expression of structural proteins located at the nuclear envelope. Through an experimental model of cell stretching and a detailed spatial intranuclear strain quantification, we identified that strain is amplified and the distribution of strain within the chromatin is altered under tensile loading in the dedifferentiated state. Further, using a confocal microscopy image-based finite element model and simulation of cell stretching, we found that the cell shape is the primary determinant of the strain amplification inside the chondrocyte nucleus in the dedifferentiated state. Additionally, we found that nuclear envelope proteins have lower gene expression in the dedifferentiated state. This study highlights the role of cell shape in nuclear mechanics and lays the groundwork to design biophysical strategies for the maintenance and enhancement of the chondrocyte phenotype during cell expansion with a goal of successful cartilage tissue engineering.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.