ArticleStructure (London, England : 1993)2022
High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells.
Article in Structure (London, England : 1993), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Subcellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging.Nature methods · 2026Article
- A calcium-binding protein-sigV regulatory circuit modulates stress adaptation and persistence in Caulobacter vibrioides.Archives of microbiology · 2026Article
- Assembly, architecture and functional roles of microbial surface layers.Nature reviews. Microbiology · 2026Review
- Donor strand complementation and calcium ion coordination drive the chaperone-free polymerization of archaeal cannulae.Nature communications · 2025Article
- Cryo-EM structure and polar assembly of the PS2 S-layer ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A self-assembling surface layer flattens the cytokinetic furrow to aid cell division in an archaeon.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A new age in structural S-layer biology: Experimental and in silico milestones.The Journal of biological chemistry · 2025Review
- Crystal structure of the C1 domain of the surface-layer protein SlpM from Lactobacillus brevis: a module involved in protein self-assembly.Acta crystallographica. Section F, Structural biology communications · 2025Article
- Mapping the ultrastructural topology of the corynebacterial cell surface.PLoS biology · 2025Article
- Elemental mapping in single-particle reconstructions by reconstructed electron energy-loss analysis.Nature methods · 2024Article
- Utilizing anomalous signals for element identification in macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2024Article
- The direct inhibitory effects of Lactobacillus acidophilus, a commensal urinary bacterium, on calcium oxalate stone development.Microbiome · 2024Article
- The parasitic lifestyle of an archaeal symbiont.Nature communications · 2024Article
- Article
- Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus.Nature communications · 2024Article
- Article
- Interdigitated immunoglobulin arrays form the hyperstable surface layer of the extremophilic bacteriumProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Synchronized Swarmers and Sticky Stalks: Caulobacter crescentus as a Model for Bacterial Cell Biology.Journal of bacteriology · 2023Review
- Article
- A multidomain connector links the outer membrane and cell wall in phylogenetically deep-branching bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
Abstract
Surface layers (S-layers) are proteinaceous crystalline coats that constitute the outermost component of most prokaryotic cell envelopes. In this study, we have investigated the role of metal ions in the formation of the Caulobacter crescentus S-layer using high-resolution structural and cell biology techniques, as well as molecular simulations. Utilizing optical microscopy of fluorescently tagged S-layers, we show that calcium ions facilitate S-layer lattice formation and cell-surface binding. We report all-atom molecular dynamics simulations of the S-layer lattice, revealing the importance of bound metal ions. Finally, using electron cryomicroscopy and long-wavelength X-ray diffraction experiments, we mapped the positions of metal ions in the S-layer at near-atomic resolution, supporting our insights from the cellular and simulations data. Our findings contribute to the understanding of how C. crescentus cells form a regularly arranged S-layer on their surface, with implications on fundamental S-layer biology and the synthetic biology of self-assembling biomaterials.
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