Evidence map›Paper›PMID 34798885›Full record

ArticleBMC veterinary research2021

Establishment of a peptide-based enzyme-linked immunosorbent assay for detecting antibodies against PRRSV M protein.

Jun Zhao, Rubo Zhang, Ling Zhu, Huidan Deng, Fengqing Li, Lei Xu, Jianbo Huan, Xiangang Sun, Zhiwen Xu

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Jun ZhaoCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Rubo ZhangCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Ling ZhuCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Huidan DengCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Fengqing LiCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Lei XuCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Jianbo HuanCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Xiangang SunCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
Zhiwen XuCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. abtcxzw@126.com.
Sichuan Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPorcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating diseases affecting the swine industry globally. Evaluation of antibody responses and neutralizing antibody titers is the most effective method for vaccine evaluation. In this study, the B cell line epitopes of PRRSV M protein were predicted, and two peptide ELISA assays were established (M-A110-129 ELISA, M-A148-174 ELISA) to detect antibodies against PRRSV M protein. Field serum samples collected from pig farms were used to validate the peptide ELISA and compare it with an indirect immunofluorescence assay.

resultsThe sensitivity and specificity of M-A110-129 ELISA and M-A148-174 ELISA were (111/125) 88.80%, (69/70) 98.57% and (122/125) 97.60%, (70/70) 100%, relative to indirect immunofluorescence assay. This peptide ELISA could detect antibodies against different genotypes of PRRSV including type 1 PRRSV, classical PRRSV, HP-PRRSV, and NADC30 like PRRSV, but not antibodies against other common swine viruses. The results of ROC analysis showed that the area under the curve (AUC) of the M-A110-129 ELISA and M-A148-174 ELISA were 0.967 and 0.996, respectively. Compared the concordance of results using two peptide ELISA assays, the IDEXX PRRSV X3 Ab ELISA and a virus neutralization test, were assessed using a series of 147 sera from pigs vaccinated with the NADC30-like PRRSV inactivated vaccine. The M-A148-174 ELISA had the best consistency, with a Cohen's kappa coefficient of 0.8772. The concordance rates of the Hipra PRRSV ELISA kit, M-A110-129 ELISA and M-A148-174 ELISA in the field seropositive detection results were 91.08, 86.32 and 95.35%, relative to indirect immunofluorescence assay.

conclusionsIn summary, compared with M-A110-129 ELISA, the PRRSV M-A148-174 ELISA is of value for detecting antibodies against PRRSV and the evaluation of the NADC30-like PRRSV inactivated vaccine, but the advantage is insufficient in serological early diagnosis.

Indexed as

AnimalsAntibodies, NeutralizingEnzyme-Linked Immunosorbent AssayFluorescent Antibody Technique, IndirectPorcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusSensitivity and SpecificitySwineVaccines, InactivatedViral Matrix ProteinsAntibodies, NeutralizingM protein, porcine reproductive and respiratory syndrome virusVaccines, InactivatedViral Matrix ProteinsEnzyme-linked immunosorbent assayM proteinNADC30-like PRRSV inactivated vaccinePigPorcine reproductive and respiratory syndrome virusSynthetic peptide

Identifiers

PMID34798885
PMCPMC8602981
OpenAlexW3214083209

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LicenceCC BY
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.