Evidence map›Paper›PMID 34797983›Full record

ArticleACS sensors2021

Fluorescent Nanodiamonds for Detecting Free-Radical Generation in Real Time during Shear Stress in Human Umbilical Vein Endothelial Cells.

Rokshana Sharmin, Thamir Hamoh, Alina Sigaeva, Aldona Mzyk, Viraj G Damle, Aryan Morita, Thea Vedelaar, Romana Schirhagl

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS sensors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 48 citations in OpenAlex.

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  9. Free radical detection in precision-cut mouse liver slices with diamond-based quantum sensing.Proceedings of the National Academy of Sciences of the United States of America · 2024
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  20. Recent Developments of Nanodiamond Quantum Sensors for Biological Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 3 countries.

Rokshana SharminDepartment Biomedical Engineering, Groningen University, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AW Groningen, The Netherlands.
Thamir HamohDepartment Biomedical Engineering, Groningen University, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AW Groningen, The Netherlands.
Alina SigaevaDepartment Biomedical Engineering, Groningen University, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AW Groningen, The Netherlands.
Aldona MzykDepartment Biomedical Engineering, Groningen University, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AW Groningen, The Netherlands.
Viraj G DamleDepartment Biomedical Engineering, Groningen University, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AW Groningen, The Netherlands.
Aryan MoritaDepartment Biomedical Engineering, Groningen University, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AW Groningen, The Netherlands.
Thea VedelaarDepartment Biomedical Engineering, Groningen University, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AW Groningen, The Netherlands.
Romana SchirhaglDepartment Biomedical Engineering, Groningen University, University Medical Center Groningen, Antonius Deusinglaan 1, 9713AW Groningen, The Netherlands.ORCID 0000-0002-8749-1054
University Medical Center Groningen · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Free-radical generation is suspected to play a key role in cardiovascular diseases. Another crucial factor is shear stress. Human umbilical vein endothelial cells (HUVECS), which form the lining of blood vessels, require a physiological shear stress to activate many vasoactive factors. These are needed for maintaining vascular cell functions such as nonthrombogenicity, regulation of blood flow, and vascular tone. Additionally, blood clots form at regions of high shear stress within a blood vessel. Here, we use a new method called diamond magnetometry which allows us to measure the dynamics of free-radical generation in real time under shear stress. This quantum sensing technique allows free-radical detection with nanoscale resolution at the single-cell level. We investigate radical formation in HUVECs in a microfluidic environment under different flow conditions typically found in veins and arteries. Here, we looked into free-radical formation before, during, and after flow. We found that the free-radical production varied depending on the flow conditions. To confirm the magnetometry results and to differentiate between radicals, we performed conventional fluorescent reactive oxygen species (ROS) assays specific for superoxide, nitric oxide, and overall ROS.

Indexed as

NanodiamondsHumansHuman Umbilical Vein Endothelial CellsNitric OxideReactive Oxygen SpeciesStress, MechanicalNanodiamondsNitric OxideReactive Oxygen SpeciescellsnanodiamondsNV centersquantum sensingrelaxometryshear stress

Identifiers

PMID34797983
PMCPMC8715866
OpenAlexW3211912611

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.