ArticleJournal of biomedical optics2021
Two-photon scanned light sheet fluorescence microscopy with axicon imaging for fast volumetric imaging.
Article in Journal of biomedical optics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 12 citations in OpenAlex.
- Imaging 3D cell cultures with optical microscopy.Nature methods · 2025Review
- Extended Depth of Focus Two-Photon Light-Sheet Microscopy for In Vivo Fluorescence Imaging of Large Multicellular Organisms at Cellular Resolution.International journal of molecular sciences · 2023Article
- Structural and functional imaging of brains.Science China. Chemistry · 2023Review
- Methods and applications of full-field optical coherence tomography: a review.Journal of biomedical optics · 2022Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
significanceTwo-photon microscopy has become the standard platform for deep-tissue fluorescence imaging. However, the use of point scanning in conventional two-photon microscopy limits the speed of volumetric image acquisition.
aimTo obtain fast and deep volumetric images, we combine two-photon light sheet fluorescence microscopy (2p-LSFM) and axicon imaging that yields an extended depth of field (DOF) in 2p-LSFM. APPROACH: Axicon imaging is achieved by imposing an axicon lens in the detection part of LSFM.
resultsThe DOF with axicon imaging is extended more than 20-fold over that of a conventional imaging lens, liberating the synchronized scanning in LSFM. We captured images of dynamic beating hearts and red blood cells in zebrafish larvae at volume acquisition rates up to 30 Hz.
conclusionsWe demonstrate the fast three-dimensional imaging capability of 2p-LSFM with axicon imaging by recording the rapid dynamics of physiological processes.
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